Whiteside T L, Lasky S, Si L, Van Thiel D H
Hepatology. 1985 May-Jun;5(3):468-74. doi: 10.1002/hep.1840050321.
Using monoclonal antibodies to surface antigens, we typed and quantitated the mononuclear cells (MNCs) infiltrating liver tissues from 23 patients with primary sclerosing cholangitis. The circulating T lymphocytes were also enumerated by flow cytometry in 12 of these patients. In blood, T lymphocytes and especially T8+ cells were decreased. T cells were the major components of MNCs (78%) in portal tracts. Few of these cells were Tac+ or HLA-DR+. Total MNCs (929 +/- 90 vs. 106 +/- 18 in controls) included 11% M1+ and 8% B1+ cells. Aggregates of T4+ and T8+ cells were common in portal areas. The portal T4/T8 ratio of 1.27 +/- 0.1 was significantly (p less than 0.01) different than that around proliferating bile ductules (0.8 +/- 0.1). The T8+ and M5+ cells localized in these areas of proliferation and infiltrated ductal epithelium. On the other hand, T4+ cells accumulated on the parenchymal side of fibrosed tissue and densely surrounded certain intralobular bile ducts. Even late in the disease, variability in the extent of MNCs infiltration, ductal proliferation and periductal fibrosis was seen in each biopsy.
我们使用针对表面抗原的单克隆抗体，对23例原发性硬化性胆管炎患者肝组织中的单核细胞（MNC）进行了分型和定量分析。其中12例患者还通过流式细胞术对循环T淋巴细胞进行了计数。血液中T淋巴细胞尤其是T8 +细胞减少。T细胞是门管区MNC的主要成分（78%）。这些细胞中很少是Tac +或HLA - DR +。总的MNC（929±90，对照组为106±18）包括11%的M1 +和8%的B1 +细胞。T4 +和T8 +细胞聚集在门管区很常见。门管区T4/T8比值为1.27±0.1，与增生性胆小管周围的比值（0.8±0.1）有显著差异（p＜0.01）。T8 +和M5 +细胞定位于这些增生区域并浸润导管上皮。另一方面，T4 +细胞聚集在纤维化组织的实质侧并密集围绕某些小叶内胆管。即使在疾病晚期，每次活检中MNC浸润程度、导管增生和导管周围纤维化的程度仍存在差异。
