Department of Food and Drug, University of Parma, Viale delle Scienze 17/A, I-43124, Parma, Italy.
Chair of Analytical Food Chemistry, Technical University of Munich, Max-von-Imhof-Forum 2, D-85354, Freising, Germany.
Sci Rep. 2019 Oct 4;9(1):14320. doi: 10.1038/s41598-019-50786-w.
The present study aimed at elucidating the uptake and biotransformation of T2 and HT2 toxins in five cultivars of durum wheat, by means of cultured plant organs. An almost complete absorption of T2 toxin (up to 100 µg) was noticed after 7 days, along with the contemporaneous formation of HT2 in planta, whereas HT2 showed a slower uptake by uninfected plant organs. Untargeted MS-analysis allowed to identify a large spectrum of phase I and phase II metabolites, resulting in 26 T2 and 23 HT2 metabolites plus tentative isomers. A novel masked mycotoxin, 3-acetyl-HT2-glucoside, was reported for the first time in wheat. The in vitro approach confirmed its potential to both investigate the contribution of plant metabolism in the biosynthesis of masked mycotoxins and to foresee the development of biocatalytic tools to develop nature-like mixtures to be used as reference materials.
本研究旨在通过培养的植物器官阐明 T2 和 HT2 毒素在五种硬质小麦品种中的吸收和生物转化。7 天后，几乎完全吸收了 T2 毒素（高达 100μg），同时在植物体内形成了 HT2，而 HT2 被未感染的植物器官吸收得更慢。非靶向 MS 分析可鉴定出大量的 I 相和 II 相代谢物，共鉴定出 26 种 T2 代谢物和 23 种 HT2 代谢物，外加可能的异构体。在小麦中首次报道了一种新型的掩蔽真菌毒素 3-乙酰基-HT2-葡萄糖苷。体外方法证实了它在研究植物代谢物在掩蔽真菌毒素生物合成中的作用以及开发生物催化工具来开发类似天然的混合物作为参考物质方面的潜力。
