Day A, Zamel N
J Appl Physiol (1985). 1985 May;58(5):1449-52. doi: 10.1152/jappl.1985.58.5.1449.
The role of the cholinergic system in the phenomenon of bronchodilatation following a deep inspiration (BDFI) in humans has not been well established, although animal studies have suggested the cholinergic system to be of prime importance. We therefore induced cholinergic blockade with inhaled ipratropium bromide (Sch-1000) in five asymptomatic subjects and then assessed whether BDFI had been abolished. Since BDFI is only evident where there is normal or increased bronchomotor tone, prostaglandin F2 alpha (PGF2 alpha), a noncholinergic bronchoconstrictor, was used to re-establish bronchomotor tone in the presence of cholinergic blockade. At each stage the presence or absence of BDFI was assessed by comparing flows from a partial forced expiratory maneuver started at approximately 60% of vital capacity (Vmaxp) with flows from a forced expiratory maneuver started at lung capacity (Vmaxc). Flows were measured at the last 40% of vital capacity. The percent ratio of Vmaxp/Vmaxc was used as an indicator of BDFI. In the presence of cholinergic blockade and with reestablishment of bronchomotor tone with PGF2 alpha, BDFI could still be demonstrated (Vmaxp/Vmaxc percent ratio: control 110.3 +/- 10.6, after Sch-1000 129.4 +/- 10.3, after Sch-1000 and PGF2 alpha 59.4 +/- 6.9; P = 0.001). We conclude that there is not an essential role for the cholinergic system in the phenomenon of BDFI in healthy individuals.
胆碱能系统在人类深吸气后支气管扩张现象（BDFI）中的作用尚未完全明确，尽管动物研究表明胆碱能系统至关重要。因此，我们对五名无症状受试者吸入异丙托溴铵（Sch - 1000）以诱导胆碱能阻滞，然后评估BDFI是否被消除。由于BDFI仅在支气管运动张力正常或增加时才明显，所以在存在胆碱能阻滞的情况下，使用非胆碱能支气管收缩剂前列腺素F2α（PGF2α）来重建支气管运动张力。在每个阶段，通过比较从大约肺活量60%开始的部分用力呼气动作（Vmaxp）的流量与从肺总量开始的用力呼气动作（Vmaxc）的流量，来评估BDFI的存在与否。流量在肺活量的最后40%进行测量。Vmaxp/Vmaxc的百分比用作BDFI的指标。在存在胆碱能阻滞且用PGF2α重建支气管运动张力的情况下，仍可证明BDFI（Vmaxp/Vmaxc百分比：对照组110.3±10.6，Sch - 1000后129.4±10.3，Sch - 1000和PGF2α后59.4±6.9；P = 0.001）。我们得出结论，胆碱能系统在健康个体的BDFI现象中并非起关键作用。
