Department of Molecular, Cellular, and Developmental Biology, University of California Santa Cruz, Santa Cruz, CA 95064, USA.
Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA 95064, USA.
Philos Trans R Soc Lond B Biol Sci. 2019 Nov 25;374(1786):20190097. doi: 10.1098/rstb.2019.0097. Epub 2019 Oct 7.
Long-read sequencing holds great potential for transcriptome analysis because it offers researchers an affordable method to annotate the transcriptomes of non-model organisms. This, in turn, will greatly benefit future work on less-researched organisms like unicellular eukaryotes that cannot rely on large consortia to generate these transcriptome annotations. However, to realize this potential, several remaining molecular and computational challenges will have to be overcome. In this review, we have outlined the limitations of short-read sequencing technology and how long-read sequencing technology overcomes these limitations. We have also highlighted the unique challenges still present for long-read sequencing technology and provided some suggestions on how to overcome these challenges going forward. This article is part of a discussion meeting issue 'Single cell ecology'.
长读测序在转录组分析中具有巨大的潜力，因为它为研究人员提供了一种经济实惠的方法来注释非模式生物的转录组。这反过来又将极大地有益于未来对单细胞真核生物等研究较少的生物体的研究，因为这些生物体不能依赖大型联合体来生成这些转录组注释。然而，要实现这一潜力，还必须克服几个仍然存在的分子和计算方面的挑战。在这篇综述中，我们概述了短读测序技术的局限性，以及长读测序技术如何克服这些局限性。我们还强调了长读测序技术仍然存在的独特挑战，并就如何克服这些挑战提出了一些建议。本文是“单细胞生态学”专题讨论的一部分。
