College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China; Tsing biomedical research center, Lanzhou University Second Hospital, Lanzhou 730030, China.
College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
Biomed Pharmacother. 2019 Dec;120:109470. doi: 10.1016/j.biopha.2019.109470. Epub 2019 Oct 4.
Fufang Xueshuantong (FXST), a Chinese patent medicine, is composed of Panax notoginseng, Salviae miltiorrhizae, Astragali Radix and Radix Scrophulariae and has been found to prevent diabetic retinopathy. Yes-associated protein (YAP) participates in the pathophysiology of retinal disease and promotes endothelial cell proliferation and angiogenesis. Although it is known that YAP activity is altered by FXST, the role of YAP in mediating the effect of FXST remains unclear. In high glucose-treated retinal vascular endothelial cells (RVECs), FXST significantly reduced cell viability, the number of migrating cells and tube length in the present study. Moreover, FXST decreased the levels of YAP mRNA and protein and inhibited the expression of vascular endothelial growth factor (VEGF). Transfection of sh-YAP into the cells decreased the ability of FXST to modulate cell migration and tube formation. The effect of FXST on VEGF expression was also decreased. Similar results were obtained when the cells were stimulated with a YAP inhibitor in combination with FXST. Thus, FXST is shown to protect high glucose-injured RVECs via YAP-mediated effects.
复方血栓通（FXST）是一种中药，由三七、丹参、黄芪和玄参组成，已被发现可预防糖尿病视网膜病变。Yes 相关蛋白（YAP）参与视网膜疾病的病理生理学过程，并促进内皮细胞增殖和血管生成。虽然已知 FXST 会改变 YAP 的活性，但 YAP 在介导 FXST 作用中的作用尚不清楚。在高糖处理的视网膜血管内皮细胞（RVECs）中，FXST 在本研究中显著降低了细胞活力、迁移细胞数量和管腔长度。此外，FXST 降低了 YAP mRNA 和蛋白水平，并抑制了血管内皮生长因子（VEGF）的表达。将 sh-YAP 转染到细胞中降低了 FXST 调节细胞迁移和管状形成的能力。FXST 对 VEGF 表达的影响也降低了。当细胞同时受到 YAP 抑制剂和 FXST 的刺激时，也得到了类似的结果。因此，FXST 通过 YAP 介导的作用来保护高糖损伤的 RVECs。
