Chen Yun-Wen, Huang Yun-Ping, Wu Pei-Chang, Chiang Wei-Yu, Wang Ping-Hsun, Chen Bo-Yie
Department of Ophthalmology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 88301, Taiwan.
Department of Optometry, Chung Shan Medical University, Taichung 40201, Taiwan.
Nutrients. 2021 Mar 17;13(3):978. doi: 10.3390/nu13030978.
Danshensu, a traditional herb-based active component ( Bunge), has garnered attention, due to its safety, nutritional value, and antioxidant effects, along with cardiovascular-protective and neuroprotective abilities; however, its effect on the retinal tissues and functional vision has not been fully studied. The objective of this study was to analyze the protective effect of danshensu on retinal tissues and functional vision in vivo in a mouse model of light-induced retinal degeneration. High energy light-evoked visual damage was confirmed by the loss in structural tissue integrity in the retina accompanied by a decline in visual acuity and visual contrast sensitivity function (VCSF), whereas the retina tissue exhibited severe Müller cell gliosis. Although danshensu treatment did not particularly reduce light-evoked damage to the photoreceptors, it significantly prevented Müller cell gliosis. Danshensu exerted protective effects against light-evoked deterioration on low spatial frequency-based VCSF as determined by the behavioral optomotor reflex method. Additionally, the protective effect of danshensu on VCSF can be reversed and blocked by the injection of a dopamine D1 receptor antagonist (SCH 23390). This study demonstrated that the major functional vision promotional effect of danshensu in vivo was through the dopamine D1 receptors enhancement pathway, rather than the structural protection of the retinas.
丹参素是一种基于传统草药的活性成分（丹参），因其安全性、营养价值、抗氧化作用以及心血管保护和神经保护能力而受到关注；然而，其对视网膜组织和功能性视力的影响尚未得到充分研究。本研究的目的是在光诱导视网膜变性小鼠模型中分析丹参素对视网膜组织和功能性视力的体内保护作用。高能光诱发的视觉损伤通过视网膜结构组织完整性的丧失以及视力和视觉对比敏感度功能（VCSF）的下降得到证实，而视网膜组织表现出严重的米勒细胞胶质增生。虽然丹参素治疗并未特别减轻光对光感受器的损伤，但它显著预防了米勒细胞胶质增生。通过行为视动反射法测定，丹参素对基于低空间频率的VCSF的光诱发退化具有保护作用。此外，注射多巴胺D1受体拮抗剂（SCH 23390）可逆转并阻断丹参素对VCSF的保护作用。本研究表明，丹参素在体内的主要功能性视力促进作用是通过多巴胺D1受体增强途径，而不是对视网膜的结构保护。
