Steeg P S, Sztein M B, Mann D L, Strong D M, Oppenheim J J
Scand J Immunol. 1985 May;21(5):425-30. doi: 10.1111/j.1365-3083.1985.tb01828.x.
Our research suggests that interferon may have an immunoregulatory role in the initiation phase of immune responses. Recent evidence has demonstrated that lymphokines regulate monocyte cell surface expression of DR antigens and, consequently, the ability of monocytes to activate T lymphocytes in an antigen-specific manner. In this report cloned interferons and a homogeneous cell line were used to demonstrate that interferon possesses these immunoregulatory functions. Cells of the promyelocytic cell line HL60, when incubated in vitro with recombinant gamma (IFN-gamma) and with alpha interferons (IFN-alpha), expressed enhanced levels of DR antigen as determined by both cytotoxicity and fluorescence-activated cell sorter analyses. Lower concentrations of IFN-gamma than IFN-alpha were needed to induce DR expression, and a higher percentage of monocytes were induced to express DR antigen by IFN-gamma than IFN-alpha. HL60 cells preincubated with lymphocyte-derived lymphokines or IFN-alpha also stimulated a significantly better in vitro allogeneic response in the mixed leukocyte reaction than untreated HL60 cells. Thus, interferon both the phenotypic expression of DR antigens of HL60 cells and their functional ability to initiate T-lymphocyte responses to an alloantigen.
我们的研究表明，干扰素可能在免疫反应的起始阶段具有免疫调节作用。最近的证据表明，淋巴因子可调节单核细胞DR抗原的细胞表面表达，从而调节单核细胞以抗原特异性方式激活T淋巴细胞的能力。在本报告中，使用克隆干扰素和一种均一的细胞系来证明干扰素具有这些免疫调节功能。早幼粒细胞系HL60细胞在体外与重组γ干扰素（IFN-γ）和α干扰素（IFN-α）一起孵育时，通过细胞毒性和荧光激活细胞分选分析测定，其DR抗原表达水平增强。诱导DR表达所需的IFN-γ浓度低于IFN-α，且与IFN-α相比，IFN-γ诱导表达DR抗原的单核细胞百分比更高。与未处理的HL60细胞相比，预先用淋巴细胞衍生的淋巴因子或IFN-α孵育的HL60细胞在混合淋巴细胞反应中也能显著促进更好的体外同种异体反应。因此，干扰素既能调节HL60细胞DR抗原的表型表达，也能调节其启动T淋巴细胞对同种异体抗原反应的功能能力。
