Ulmer A J, Flad H D
Immunology. 1979 Oct;38(2):393-9.
A one-stage micro-culture assay in glass capillary tubes was developed for colony growth of human T lymphocytes. Colony growth by this method did not depend on the addition of growth factors, human serum and erythrocytes. Advantages of this method are its low consumption of materials and the fact that high numbers of cultures can be prepared and evaluated within a relatively short period of time. Compared to the two-stage method, the one-stage method requires a higher number of seeded cells and a reduced agar concentration, suggesting that cell interactions are required for human T-lymphocyte colony formation.
为了实现人T淋巴细胞的集落生长，开发了一种在玻璃毛细管中进行的单阶段微培养测定法。通过这种方法的集落生长不依赖于生长因子、人血清和红细胞的添加。该方法的优点是材料消耗低，并且能够在相对较短的时间内制备和评估大量培养物。与两阶段法相比，单阶段法需要更多的接种细胞和更低的琼脂浓度，这表明人T淋巴细胞集落形成需要细胞间相互作用。
