Ulmer A J, Flad H D
Biomedicine. 1981 Dec;34(4):192-8.
The effect of X-irradiation on growth of T-lymphocyte colony forming units (TL-CFU) from human peripheral blood was investigated. The results indicate that not the number of activated TL-CFU but the number of cell cycles of colony forming cells was reduced by X-irradiation. Therefore we presume that TL-CFU belong to a relatively radio-resistant cell population within the PHA-responsive lymphocytes. Kinetic studies revealed that colony growth following irradiation was delayed mainly during the phase of the first cell cycle. Preculture of the cells for 24 hours after irradiation with 1,200 R in the absence of PHA caused total inhibition of colony growth in the subsequent agar culture. In the presence of PHA no inhibition was observed. This finding appears to reflect a repair mechanism from radiation damage of lymphocytes stimulated by PHA.
研究了X射线照射对人外周血T淋巴细胞集落形成单位（TL-CFU）生长的影响。结果表明，X射线照射减少的不是活化TL-CFU的数量，而是集落形成细胞的细胞周期数。因此，我们推测TL-CFU属于PHA反应性淋巴细胞中相对抗辐射的细胞群体。动力学研究表明，照射后的集落生长主要在第一个细胞周期阶段延迟。在无PHA的情况下，用1200伦琴照射后将细胞预培养24小时，会导致随后琼脂培养中的集落生长完全受到抑制。在有PHA存在的情况下未观察到抑制作用。这一发现似乎反映了PHA刺激的淋巴细胞辐射损伤的修复机制。
