使用T淋巴细胞集落测定法来测量单核吞噬细胞功能。
The use of a T lymphocyte colony assay to measure mononuclear phagocyte function.
作者信息
Gordon M Y, King J A, Gordon-Smith E C
出版信息
Clin Exp Immunol. 1980 Oct;42(1):156-61.
The control of colony formation of normal PHA-stimulated lymphocytes has been investigated using a liquid-on-agar culture system (Lowenberg & De Zeeuw, 1979). Optimal colony formation requires the presence of mononuclear cells which were characterized by their ability to adhere to plastic surfaces. This finding suggests that the assay system can be used to estimate the co-operative function of mononuclear phagocytes as well as to enumerate T lymphocyte colony-forming cells (TL-CFC). Both the capacities of the mononuclear cells to promote colony formation and the colony-forming abilities of T cells varied widely between different normal individuals. Studies in patients with haemopoietic deficiencies showed that the growth of TL-CFC can be used to distinguish between a real lack of colony-forming cells and an apparent reduction, secondary to mononuclear cell depletion.
利用液体覆盖琼脂培养系统（Lowenberg和De Zeeuw，1979年）对正常PHA刺激淋巴细胞的集落形成控制进行了研究。最佳集落形成需要存在单核细胞，这些单核细胞的特征是具有粘附于塑料表面的能力。这一发现表明，该检测系统可用于评估单核吞噬细胞的协同功能以及计数T淋巴细胞集落形成细胞（TL-CFC）。不同正常个体之间，单核细胞促进集落形成的能力和T细胞的集落形成能力差异很大。对造血功能缺陷患者的研究表明，TL-CFC的生长可用于区分真正缺乏集落形成细胞和继发于单核细胞耗竭的明显减少。
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