Dey Sucharita, Sukul Pradip Kr
Department of Chemistry, Amity Institute of Applied Sciences, Amity University Kolkata, Action Area-II, Kadampukur, New Town, Rajarhat, West Bengal 700135, India.
ACS Omega. 2019 Sep 20;4(14):16191-16200. doi: 10.1021/acsomega.9b02405. eCollection 2019 Oct 1.
A water-soluble perylene diimide, aspartic acid-functionalized perylene diimide (APDI), has shown significant sequential "turn-off" and "turn-on" responses toward Cu and inorganic pyrophosphate (PPi), respectively. APDI was found to show selectivity toward Cu and inorganic PPi over adenosine monophosphate, adenosine diphosphate, and adenosine triphosphate. The detection has been studied by absorption and emission spectroscopy techniques. Incorporation of Cu into the solution of APDI results in a distinct quenching of the fluorescence intensity, while there was no spectral change in the presence of other metal ions. The formed APDI-Cu ensemble can turn on its fluorescence signal when PPi is present. The detection of PPi could be traced by looking at the change in color of the solution under the naked eye. No interference was observed from other anions, making the APDI-Cuaggregate a highly selective biosensor for PPi.
一种水溶性苝二酰亚胺，天冬氨酸功能化苝二酰亚胺（APDI），已分别对铜和无机焦磷酸（PPi）表现出显著的顺序“关断”和“开启”响应。研究发现，APDI对铜和无机PPi的选择性高于单磷酸腺苷、二磷酸腺苷和三磷酸腺苷。通过吸收和发射光谱技术对该检测进行了研究。将铜加入APDI溶液中会导致荧光强度明显猝灭，而在其他金属离子存在的情况下光谱没有变化。当存在PPi时，形成的APDI-Cu聚集体可以开启其荧光信号。通过肉眼观察溶液颜色的变化可以追踪PPi的检测。未观察到其他阴离子的干扰，这使得APDI-Cu聚集体成为一种对PPi具有高度选择性的生物传感器。
