Cubas-Atienzar Ana Isabel, Hide Geoff, Smith Judith Elisabeth
Biomedical Research Center, School of Environment and Life Sciences, University of Salford, Salford M5 4WT, U.K.
J Parasitol. 2019 Oct;105(5):738-747.
is a zoonotic parasite of worldwide distribution. The consumption of infected pork meat has been suggested to be an important source for human infection in the tropical area of Yucatan, Mexico. We performed a cross-sectional study of 12 farms across the state to investigate the seroprevalence of infection in domestic pigs. In total, 632 samples were obtained from 2 different environmental zones (tropical deciduous low forest and tropical sub-deciduous medium forest) and 2 abattoirs. The modified agglutination test (MAT) was used to assess the seroprevalence of in pigs and to evaluate 2 globally used serological tests, the Dye test (DT) and ID Screen® ELISA multi-species, and a commercial ELISA kit (Human Toxo IgG, Human-diagnostics), which is widely used locally in this geographical area. The overall prevalence obtained with the MAT (cut-off ≥1:25) among the 632 pigs was 1.4% (95% CI, 0.6-2.7%). The seroprevalence obtained for the different age groups was 0.6%, 0.7%, 1.8%, and 6.8% among 2-3, 3-4, 4-5, and ≥5-mo-old pigs. This increase in the seroprevalence was statistically significant for the 2 older groups (odds ratio [OR] 3.9-7.1, < 0.05) in comparison with younger groups. DT at >4 IU dilution had a perfect agreement and 100% of sensitivity and specificity when compared with the MAT. Although ID Screen® had only a fair agreement (κ = 0.389) with the MAT, the McNemar test showed that the results of these tests were comparable ( = 0.29). The Human Toxo ELISA showed no agreement with MAT, ID Screen®, and DT (κ = 0.000-0.023, McNemar < 0.05). This ELISA was lacking in specificity, accuracy, and precision; hence, we do not recommend its use for diagnosis in pig serum.
是一种分布于全球的人畜共患寄生虫。在墨西哥尤卡坦半岛的热带地区，食用受感染的猪肉被认为是人类感染的重要来源。我们对该州的12个农场进行了一项横断面研究，以调查家猪感染的血清阳性率。总共从2个不同的环境区域（热带落叶低地森林和热带次落叶中林）和2个屠宰场采集了632份样本。采用改良凝集试验（MAT）评估猪的血清阳性率，并评估2种全球使用的血清学检测方法，即染料试验（DT）和ID Screen® ELISA多物种检测，以及一种在该地理区域本地广泛使用的商业ELISA试剂盒（人弓形虫IgG，Human-diagnostics）。在632头猪中，MAT（临界值≥1:25）得出的总体阳性率为1.4%（95%可信区间，0.6 - 2.7%）。不同年龄组的血清阳性率在2 - 3月龄、3 - 4月龄、4 - 5月龄和≥5月龄的猪中分别为0.6%、0.7%、1.8%和6.8%。与较年轻组相比，两个较年长组的血清阳性率增加具有统计学意义（优势比[OR] 3.9 - 7.1，P < 0.05）。与MAT相比，稀释度>4 IU的DT具有完全一致性，敏感性和特异性均为100%。虽然ID Screen®与MAT仅具有中等一致性（κ = 0.389），但McNemar检验表明这些检测结果具有可比性（P = 0.29）。人弓形虫ELISA与MAT、ID Screen®和DT均无一致性（κ = 0.000 - 0.023，McNemar P < 0.05）。该ELISA缺乏特异性、准确性和精密度；因此，我们不建议将其用于猪血清中弓形虫的诊断。
