Poultry Institute, Chinese Academy of Agriculture Science, Yangzhou, Jiangsu, 225125, China; Institute of Effective Evaluation of Feed and Feed Additive (Poultry Institute), Ministry of Agriculture, Yangzhou, Jiangsu, 225125, China.
Poultry Institute, Chinese Academy of Agriculture Science, Yangzhou, Jiangsu, 225125, China; Institute of Effective Evaluation of Feed and Feed Additive (Poultry Institute), Ministry of Agriculture, Yangzhou, Jiangsu, 225125, China.
Theriogenology. 2020 Jan 15;142:120-130. doi: 10.1016/j.theriogenology.2019.09.040. Epub 2019 Sep 27.
Daidzein (DA) is a kind of isoflavone that is extracted primarily from soy plants and that has become increasingly popular as a dietary supplement. The objective of this study was to evaluate the effects of dietary DA supplementation for laying breeder hens on laying performance, reproductive organ development, hatching performance of seed eggs, and growth performance of offspring and to investigate the underlying molecular mechanisms. A total of 180 55-week-old laying breeder hens were randomly divided into 2 treatment groups and, after 3 weeks of acclimation, were fed either a control diet (CON) or a DA-supplemented diet (DAS, CON+30 mg/kg DA) for a total of 12 weeks. DAS treatment improved the laying rate, luteinizing hormone (LH) levels, and small yellow follicle (SYF) numbers without negative effects on the hatchability of breeder eggs or the growth performance of offspring. High-throughput RNA sequencing was utilized to identify differentially expressed genes in the SYF granulosa layer in the two groups. Transcriptome analysis showed that 161 genes (fold change ≥2 or ≤0.5; P-value<0.05) were significantly differentially expressed between the two groups, including 139 upregulated genes and 22 downregulated genes. Gene ontology (GO) functional annotation analysis revealed potential genes, processes and pathways involved in cell proliferation and differentiation related to the improvement of laying performance stimulated by DA. Dietary DA supplementation for laying breeder hens improved laying performance and reproductive performance with no negative impacts on hatchability or offspring growth. A series of differentially expressed genes in SYF granulosa cells were significantly upregulated in the DAS group relative to the CON group. This study provides insight into the genetic architecture of the transcriptome of the SYF granulosa layer in layer breeding hens and proposes candidate genes that respond to dietary DA.
染料木黄酮（DA）是一种异黄酮，主要从大豆植物中提取，作为膳食补充剂越来越受欢迎。本研究旨在评估日粮 DA 补充对产蛋种母鸡的产蛋性能、生殖器官发育、种蛋孵化性能和后代生长性能的影响，并探讨其潜在的分子机制。将 180 只 55 周龄的产蛋种母鸡随机分为 2 个处理组，经过 3 周的适应期后，分别饲喂对照日粮（CON）或 DA 补充日粮（DAS，CON+30mg/kg DA），共 12 周。DAS 处理提高了产蛋率、促黄体激素（LH）水平和小黄卵泡（SYF）数量，而对种蛋孵化率或后代生长性能没有负面影响。利用高通量 RNA 测序鉴定两组 SYF 颗粒层中差异表达的基因。转录组分析显示，两组间有 161 个基因（fold change≥2 或≤0.5；P-value<0.05）差异显著表达，包括 139 个上调基因和 22 个下调基因。基因本体（GO）功能注释分析揭示了与 DA 刺激产蛋性能提高相关的细胞增殖和分化的潜在基因、过程和途径。日粮 DA 补充对产蛋种母鸡的产蛋性能和繁殖性能有改善作用，对孵化率或后代生长没有负面影响。DAS 组 SYF 颗粒细胞中的一系列差异表达基因明显上调。本研究为层鸡 SYF 颗粒层转录组的遗传结构提供了新的见解，并提出了对膳食 DA 有反应的候选基因。
