RNA 编辑全景揭示了对精子发生中基因调控动态的新见解。
Landscape of RNA editing reveals new insights into the dynamic gene regulation of spermatogenesis.
机构信息
College of Life Sciences, Northwest A&F University, Yangling, China.
College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F University, Yangling, China.
出版信息
Cell Cycle. 2019 Dec;18(23):3351-3364. doi: 10.1080/15384101.2019.1676584. Epub 2019 Oct 9.
Spermatogenesis is an important physiological process associated with male infertility. As a kind of post-transcriptional regulation, RNA editings (REs) change the genetic information at the mRNA level. But whether there are REs and what's the role of REs during the process are still unclear. In this study, we integrated published RNA-Seq datasets and established a landscape of RNA REs during the development of mouse spermatogenesis. Totally, 7530 editing sites occurred in 2012 genes among all types of male germ cells were found, these sites enrich on some regions of chromosomes, including chromosome 17 and both ends of chromosome Y. We also found about half of the REs in CDSs can cause amino acids changes. Some non-synonymous REs which exist in specific genes may play important roles in spermatogenesis. Finally, we verified a non-synonymous A-to-I RNA editing site in and a stoploss editing in during spermatogenesis. In short, we systematically analyzed the dynamic landscape of RNA editing at different stages of spermatogenesis.
精子发生是与男性不育相关的重要生理过程。作为一种转录后调控，RNA 编辑（REs）改变 mRNA 水平的遗传信息。但是，在这个过程中是否存在 REs 以及它们的作用是什么仍然不清楚。在这项研究中，我们整合了已发表的 RNA-Seq 数据集，建立了小鼠精子发生过程中 RNA 编辑的全景图。总共在所有类型的雄性生殖细胞中发现了 2012 个基因中的 7530 个编辑位点，这些位点富集在染色体的某些区域，包括染色体 17 和染色体 Y 的两端。我们还发现，CDS 中的大约一半的 REs 可以导致氨基酸的变化。存在于特定基因中的一些非同义 REs 可能在精子发生中发挥重要作用。最后，我们在精子发生过程中验证了 中的一个非同义 A-to-I RNA 编辑位点和 中的一个终止损失编辑。总之，我们系统地分析了精子发生不同阶段的 RNA 编辑的动态全景。
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