College of Chemistry, Jilin University, Changchun, 130012, PR China.
College of Medical Engineering, Jining Medical University, Jining, 272067, PR China.
Talanta. 2020 Jan 15;207:120315. doi: 10.1016/j.talanta.2019.120315. Epub 2019 Sep 6.
In this paper, silver nanoclusters (AgNCs) were synthesized by one facile, low-cost and green method with histidine acting as the stabilizer and ascorbic acid performing as reducing agent. AgNCs displayed excellent fluorescence property and good biocompatibility and were further functionalized by β-cyclodextrin (CD-AgNCs) for the highly sensitive detection of alkaline phosphatase (ALP) activity. ALP catalyzed the dephosphorylation of p-nitrophenylphosphate (NPP) to produce p-nitrophenol (NP), and NP can effectively quench the fluorescence of CD-AgNCs via the inner filter effect (IFE) due to the UV-vis absorption spectrum of NP had considerable overlap with the excitation spectrum of CD-AgNCs. Benefiting from the IFE and the host-guest interaction between CD and NP, this CD-AgNCs based system for ALP activity analysis displayed a good linear range of 0.02-10.0 U L and the detection limit as low as 0.0046 U L. In addition, this proposed strategy also showed satisfactory performance for ALP analysis in human serum samples and bioimaging of intracellular ALP in living cells, indicating the great application prospect of this system for ALP activity analysis in medical related researches.
本文采用一种简便、经济、绿色的方法合成了银纳米簇（AgNCs），其中组氨酸作为稳定剂，抗坏血酸作为还原剂。AgNCs 具有优异的荧光性能和良好的生物相容性，进一步通过β-环糊精（CD-AgNCs）进行功能化，用于高灵敏度检测碱性磷酸酶（ALP）活性。ALP 催化 p-硝基苯磷酸酯（NPP）去磷酸化生成对硝基苯酚（NP），由于 NP 的紫外-可见吸收光谱与 CD-AgNCs 的激发光谱有相当大的重叠，NP 可以通过内滤效应（IFE）有效地猝灭 CD-AgNCs 的荧光。得益于 IFE 和 CD 与 NP 之间的主客体相互作用，该基于 CD-AgNCs 的 ALP 活性分析系统在 0.02-10.0 U L 的线性范围内表现出良好的性能，检测限低至 0.0046 U L。此外，该策略在人血清样品中的 ALP 分析和活细胞内 ALP 的生物成像中也表现出令人满意的性能，表明该系统在医学相关研究中用于 ALP 活性分析具有广阔的应用前景。
