Noory Parastoo, Navid Shadan, Zanganeh Bagher Minaee, Talebi Ali, Borhani-Haghighi Maryam, Gholami Keykavos, Manshadi Marjan Dehghan, Abbasi Mehdi
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Anatomy, Gonabad University of Medical Sciences, Gonabad, Iran.
Cell Reprogram. 2019 Oct;21(5):249-259. doi: 10.1089/cell.2019.0020.
We recently reported the application of human menstrual blood stem cells' (HuMenSCs) transplantation as a treatment modality in a rat model of premature ovarian failure (POF). We continued to investigate further in this respect. Female rats were injected intraperitoneally with 36 mg/kg busulfan. HuMenSCs were obtained, grown, and analyzed for immunophenotypic features at passage three. The cells were labeled with CM-Dil and infused into the rats. There were four groups: normal, negative control, treatment, and Sham. One month after treatment, the ovaries were collected and weighed. Histological sections were prepared from the ovary and HuMenSCs were tracking. Subsequently, we examined the changes of expression of Bax and B cell lymphoma 2 (Bcl2) genes by real-time polymerase chain reaction assay. One month after HuMenSCs transplantation, these cells were located in the ovarian interstitium and granulosa cells (GCs). The number of TUNEL-positive cells significantly decreased in the treatment group. Also the expression level of Bax genes, unlike Bcl2 gene, significantly decreased compared with negative and sham groups. In our study, HuMenSCs were tracked in ovarian tissues within 2 months after transplantation, and they differentiated into GCs. Therefore, the use of these cells can be a practical and low-cost method for the treatment of POF patients.
我们最近报道了将人月经血干细胞（HuMenSCs）移植作为一种治疗方式应用于大鼠卵巢早衰（POF）模型。我们继续对此进行进一步研究。给雌性大鼠腹腔注射36 mg/kg白消安。获取HuMenSCs，在传代三次时进行培养并分析其免疫表型特征。用CM-Dil标记细胞并注入大鼠体内。有四组：正常组、阴性对照组、治疗组和假手术组。治疗一个月后，收集卵巢并称重。从卵巢制备组织切片并追踪HuMenSCs。随后，通过实时聚合酶链反应检测法检测Bax和B细胞淋巴瘤2（Bcl2）基因表达的变化。HuMenSCs移植一个月后，这些细胞位于卵巢间质和颗粒细胞（GCs）中。治疗组中TUNEL阳性细胞数量显著减少。与阴性和假手术组相比，Bax基因的表达水平也显著降低，而Bcl2基因则不同。在我们的研究中，HuMenSCs在移植后2个月内在卵巢组织中被追踪到，并且它们分化为GCs。因此，使用这些细胞可能是一种治疗POF患者的实用且低成本的方法。
