PRR14 过表达通过 AKT 通路促进结肠癌的细胞生长、上皮间质转化和转移。
PRR14 overexpression promotes cell growth, epithelial to mesenchymal transition and metastasis of colon cancer via the AKT pathway.
机构信息
Joint International Research Laboratory of Reproduction and Development of the Ministry of Education, Department of Reproductive Biology, Chongqing Medical University, Chongqing, China.
Medical Research Center, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China.
出版信息
PLoS One. 2019 Oct 9;14(10):e0218839. doi: 10.1371/journal.pone.0218839. eCollection 2019.
BACKGROUND
PRR14 (Proline rich protein 14) was firstly identified for its ability to specify and localize heterochromatin during cell cycle progression. Aberrant expression of PRR14 is associated with the tumorigenesis and progression of lung cancer. However, its involvement in colon cancer remains unknown. Herein, we report the role of PRR14 in colon cancer.
METHODS
Colon cancer tissue microarray was used to analyze and compare the expression of PRR14 among some clinicopathological characteristics of colon cancer. HCT116 and RKO cells were transfected with siRNA to downregulate PRR14 expression. The roles of PRR14 in proliferation, migration and invasion of the cell lines were determined using cell counting kit-8, colony formation assay, wound healing assay and transwell assays respectively. The expression of PRR14 was measured using immunofluorescence, qRT- PCR and western blot. Epithelial-mesenchymal transition (EMT) markers were determined by western blot.
RESULTS
PRR14 was highly expressed in colon cancer tissues, and the expression level was correlated with tumor size, distant metastasis and Tumor Node Metastasis stages. Functional study revealed that downregulation of PRR14 inhibited colon cancer cells growth, migration and invasion. Furthermore, knockdown of PRR14 inhibited epithelial-mesenchymal transition (EMT) process, cell cycle-associated proteins expression and p-AKT level.
CONCLUSION
PRR14 may promote the progression and metastasis of colon cancer, and may be a novel prognostic and therapeutic marker for the disease.
背景
PRR14（富含脯氨酸蛋白 14）最初因其在细胞周期进程中特异性和定位异染色质的能力而被鉴定。PRR14 的异常表达与肺癌的发生和进展有关。然而，其在结肠癌中的作用尚不清楚。在此，我们报告 PRR14 在结肠癌中的作用。
方法
使用结肠癌组织微阵列分析和比较 PRR14 在结肠癌某些临床病理特征中的表达。用 siRNA 转染 HCT116 和 RKO 细胞以下调 PRR14 表达。使用细胞计数试剂盒-8、集落形成实验、划痕愈合实验和 Transwell 实验分别确定 PRR14 在细胞系增殖、迁移和侵袭中的作用。使用免疫荧光、qRT-PCR 和 Western blot 测量 PRR14 的表达。通过 Western blot 测定上皮-间充质转化（EMT）标志物。
结果
PRR14 在结肠癌组织中高表达，表达水平与肿瘤大小、远处转移和肿瘤淋巴结转移分期相关。功能研究表明，下调 PRR14 抑制结肠癌细胞生长、迁移和侵袭。此外，敲低 PRR14 抑制上皮-间充质转化（EMT）过程、细胞周期相关蛋白表达和 p-AKT 水平。
结论
PRR14 可能促进结肠癌的进展和转移，可能是该疾病的一种新的预后和治疗标志物。
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