Reyes-Ortega Felisa, Checa Fernández Blanca Luna, Delgado Angel V, Iglesias Guillermo R
Department of Applied Physics, School of Sciences, University of Granada, 18071 Granada, Spain.
Pharmaceutics. 2019 Oct 8;11(10):517. doi: 10.3390/pharmaceutics11100517.
In this paper, it is proposed that polymer-coated magnetic nanorods (MNRs) can be used with the advantage of a double objective: first, to serve as magnetic hyperthermia agents, and second, to be used as magnetic vehicles for the antitumor drug doxorubicin (DOX). Two different synthetic methodologies (hydrothermal and co-precipitation) were used to obtain MNRs of maghemite and magnetite. They were coated with poly(ethyleneimine) and poly(sodium 4-styrenesulfonate), and loaded with DOX, using the Layer-by-Layer technique. Evidence of the polymer coating and the drug loading was justified by ATR-FTIR and electrophoretic mobility measurements, and the composition of the coated nanorods was obtained by a thermogravimetric analysis. The nanorods were tested as magnetic hyperthermia agents, and it was found that they provided sufficiently large heating rates to be used as adjuvant therapy against solid tumors. DOX loading and release were determined by UV-visible spectroscopy, and it was found that up to 50% of the loaded drug was released in about 5 h, although the rate of release could be regulated by simultaneous application of hyperthermia, which acts as a sort of external release-trigger. Shape control offers another physical property of the particles as candidates to interact with tumor cells, and particles that are not too elongated can easily find their way through the cell membrane.
本文提出，聚合物包覆的磁性纳米棒（MNRs）可用于实现双重目标：其一，用作磁热疗剂；其二，用作抗肿瘤药物阿霉素（DOX）的磁性载体。采用两种不同的合成方法（水热法和共沉淀法）制备了磁赤铁矿和磁铁矿的MNRs。使用层层技术，用聚乙烯亚胺和聚（4-苯乙烯磺酸钠）对其进行包覆，并负载DOX。通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）和电泳迁移率测量证明了聚合物包覆和药物负载情况，并通过热重分析获得了包覆纳米棒的组成。对纳米棒作为磁热疗剂进行了测试，发现它们提供了足够大的加热速率，可用作实体瘤的辅助治疗。通过紫外可见光谱法测定了DOX的负载和释放情况，发现高达50%的负载药物在约5小时内释放，不过释放速率可通过同时施加热疗来调节，热疗起到一种外部释放触发的作用。形状控制为颗粒提供了另一种与肿瘤细胞相互作用的物理特性，不太细长的颗粒能够轻松穿过细胞膜。
