尼龙棉柱分离引起的淋巴细胞膜改变。
Lymphocyte membrane alterations caused by nylon wool column separation.
作者信息
Corrigan A, O'Kennedy R, Smyth H
出版信息
J Immunol Methods. 1979;31(1-2):177-82. doi: 10.1016/0022-1759(79)90298-9.
PMID:315973
Abstract
Use of the nylon wool column method to separate lymphocytes results in preparations which, while fully viable, have grossly depleted cellular levels of phospholipid, cholesterol and sialic acid. These membrane effects, which relate mainly to T cells, indicate that the method is unsuitable for use in biochemical analysis of lymphocytes.
摘要
使用尼龙毛柱法分离淋巴细胞会得到这样的制剂，虽然细胞完全存活，但磷脂、胆固醇和唾液酸的细胞水平已大幅降低。这些主要与T细胞相关的膜效应表明该方法不适用于淋巴细胞的生化分析。
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