Cell Polarity, Migration and Cancer Unit, Institut Pasteur, UMR3691 CNRS, Equipe Labellisée Ligue Contre le Cancer, 75015 Paris, France.
Université Paris Descartes, Center for Research and Interdisciplinarity, Sorbonne Paris Cité, 12 Rue de l'École de Médecine, 75006 Paris, France.
J Cell Sci. 2019 Oct 9;132(19):jcs232843. doi: 10.1242/jcs.232843.
Cell adhesion to the extracellular matrix is essential for cellular processes, such as migration and invasion. In response to cues from the microenvironment, integrin-mediated adhesions alter cellular behaviour through cytoskeletal rearrangements. The tight association of the actin cytoskeleton with adhesive structures has been extensively studied, whereas the microtubule network in this context has gathered far less attention. In recent years, however, microtubules have emerged as key regulators of cell adhesion and migration through their participation in adhesion turnover and cellular signalling. In this Review, we focus on the interactions between microtubules and integrin-mediated adhesions, in particular, focal adhesions and podosomes. Starting with the association of microtubules with these adhesive structures, we describe the classical role of microtubules in vesicular trafficking, which is involved in the turnover of cell adhesions, before discussing how microtubules can also influence the actin-focal adhesion interplay through RhoGTPase signalling, thereby orchestrating a very crucial crosstalk between the cytoskeletal networks and adhesions.
细胞与细胞外基质的黏附对于细胞的迁移和侵袭等过程是必不可少的。细胞整合素介导的黏附通过细胞骨架重排来改变细胞行为，以响应微环境中的信号。细胞骨架与黏附结构的紧密关联已经得到了广泛的研究，而微管网络在这方面的研究则相对较少。然而，近年来，微管通过参与黏附的周转和细胞信号转导，已成为细胞黏附和迁移的关键调节因子。在这篇综述中，我们重点讨论了微管与整合素介导的黏附（特别是黏着斑和伪足）之间的相互作用。我们首先描述了微管与这些黏附结构的关联，然后讨论了微管如何通过 Rho GTPase 信号影响肌动蛋白-黏着斑的相互作用，从而协调细胞骨架网络和黏附之间的这种非常关键的串扰。接着，我们描述了微管在囊泡运输中的经典作用，囊泡运输参与了细胞黏附的周转，最后讨论了微管如何通过 Rho GTPase 信号影响肌动蛋白-黏着斑的相互作用，从而协调细胞骨架网络和黏附之间的这种非常关键的串扰。
