Phi Cong Nguyen, Fujita Daisuke, Yamagata Yoshiyuki, Yoshimura Atsushi, Yasui Hideshi
Plant Breeding Laboratory, Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Breed Sci. 2019 Sep;69(3):439-446. doi: 10.1270/jsbbs.19029. Epub 2019 Jun 27.
The green rice leafhopper (GRH), Uhler, is a major insect pest of cultivated rice, L., throughout the temperate regions of East Asia. GRH resistance had been reported in the wild species but genetic basis of GRH resistance in wild rice accession has not been clarified. Here, we found a major QTL, , on chromosome 4 conferred GRH resistance with 14.1 of the logarithm of odds (LOD) score explaining 67.6% of phenotypic variance in the BCF population derived from a cross between the susceptible cultivar 'Taichung 65' (T65) and accession IRGC105715. has been identified as between the markers RM5414 and C60248 in a BCF population derived from two BCF plants resistant to GRH. In a high-resolution mapping, the region was delimited between the markers G6-c60k and 7L16f, and corresponded to an 31.2-kbp region of the 'Nipponbare' genome. Understanding the genetic basis of GRH resistance will facilitate the use of GRH resistance genes in marker-assisted breeding in rice.
黑尾叶蝉（Uhler）是东亚温带地区栽培稻（L.）的主要害虫。野生稻种中已报道有抗黑尾叶蝉的情况，但野生稻种质资源中抗黑尾叶蝉的遗传基础尚未明确。在此，我们在第4号染色体上发现了一个主效QTL，其对数似然比（LOD）得分为14.1，解释了来自感虫品种‘台中65’（T65）与种质资源IRGC105715杂交产生的BCF群体中67.6%的表型变异，该QTL赋予了对黑尾叶蝉的抗性。在来自两株抗黑尾叶蝉的BCF植株的BCF群体中，该QTL已被定位在标记RM5414和C60248之间。在高分辨率定位中，该QTL区域被限定在标记G6 - c60k和7L16f之间，对应于‘日本晴’基因组的一个31.2千碱基对的区域。了解抗黑尾叶蝉的遗传基础将有助于在水稻的标记辅助育种中利用抗黑尾叶蝉基因。
