水稻品种‘ASD7’（Oryza sativa L. ssp. indica）对褐飞虱（Nilaparvata lugens Stål）和绿稻叶蝉（Nephotettix cincticeps Uhler）多重抗性的遗传基础。

Genetic basis of multiple resistance to the brown planthopper (Nilaparvata lugens Stål) and the green rice leafhopper (Nephotettix cincticeps Uhler) in the rice cultivar 'ASD7' (Oryza sativa L. ssp. indica).

作者信息

Van Mai Tan, Fujita Daisuke, Matsumura Masaya, Yoshimura Atsushi, Yasui Hideshi

机构信息

Plant Breeding Laboratory, Faculty of Agriculture, Graduate School, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan.

Plant Breeding Laboratory, Faculty of Agriculture, Graduate School, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan ; Global Human Resources Development Project, Faculty of Agriculture, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan.

出版信息

Breed Sci. 2015 Dec;65(5):420-9. doi: 10.1270/jsbbs.65.420. Epub 2015 Dec 1.

The rice cultivar ASD7 (Oryza sativa L. ssp. indica) is resistant to the brown planthopper (BPH; Nilaparvata lugens Stål) and the green leafhopper (Nephotettix virescens Distant). Here, we analyzed multiple genetic resistance to BPH and the green rice leafhopper (GRH; Nephotettix cincticeps Uhler). Using two independent F2 populations derived from a cross between ASD7 and Taichung 65 (Oryza sativa ssp. japonica), we detected two QTLs (qBPH6 and qBPH12) for resistance to BPH and one QTL (qGRH5) for resistance to GRH. Linkage analysis in BC2F3 populations revealed that qBPH12 controlled resistance to BPH and co-segregated with SSR markers RM28466 and RM7376 in plants homozygous for the ASD7 allele at qBPH6. Plants homozygous for the ASD7 alleles at both QTLs showed a much faster antibiosis response to BPH than plants homozygous at only one of these QTLs. It revealed that epistatic interaction between qBPH6 and qBPH12 is the basis of resistance to BPH in ASD7. In addition, qGRH5 controlled resistance to GRH and co-segregated with SSR markers RM6082 and RM3381. qGRH5 is identical to GRH1. Thus, we clarified the genetic basis of multiple resistance of ASD7 to BPH and GRH.

水稻品种ASD7（Oryza sativa L. ssp. indica）对褐飞虱（BPH；Nilaparvata lugens Stål）和绿叶蝉（Nephotettix virescens Distant）具有抗性。在此，我们分析了对褐飞虱和绿稻叶蝉（GRH；Nephotettix cincticeps Uhler）的多重遗传抗性。利用来自ASD7与台中65（Oryza sativa ssp. japonica）杂交的两个独立F2群体，我们检测到两个抗褐飞虱的QTL（qBPH6和qBPH12）以及一个抗绿稻叶蝉的QTL（qGRH5）。BC2F3群体中的连锁分析表明，qBPH12控制对褐飞虱的抗性，并在qBPH6位点上对ASD7等位基因纯合的植株中与SSR标记RM28466和RM7376共分离。在两个QTL上对ASD7等位基因均纯合的植株对褐飞虱的抗生反应比仅在其中一个QTL上纯合的植株快得多。这表明qBPH6和qBPH12之间的上位性互作是ASD7对褐飞虱抗性的基础。此外，qGRH5控制对绿稻叶蝉的抗性，并与SSR标记RM6082和RM3381共分离。qGRH5与GRH1相同。因此，我们阐明了ASD7对褐飞虱和绿稻叶蝉多重抗性的遗传基础。