Qin Jing, Wang Wangsheng, An Fangmei, Huang Wei, Ding Junli
Department of Medical Oncology, Zhejiang Cancer Hospital, Hangzhou, 310022, P.R. China.
Department of Oncology, Wuxi Hospital of traditional Chinese Medicine, Wuxi, 214071, P.R. China.
J Cancer. 2019 Aug 27;10(20):4939-4946. doi: 10.7150/jca.27616. eCollection 2019.
: The lack of effective therapeutic targets poses a leading challenge to prolong survival and improve the quality of life for pancreatic cancer patients. Proteasome 26S subunit ATPase 2 (), a recently discovered gene, has been implicated in certain human carcinogenesis. However, limited data is available concerning the functional significance of in cancer cell growth, and whether it plays a role in pancreatic carcinogenesis remains unknown. : Quantitative RT-PCR (qRT-PCR) was performed to assess mRNA expression levels of in different pancreatic cancer cell lines. Knockdown of was achieved by using short hairpin RNA (shRNA). The effects of silencing on pancreatic cancer cell proliferation and apoptosis were evaluated by the MTT cell proliferation assay, Celigoassays, Annexin V fluorescence-activated cell sorting (FACS) assay and Caspase-3/7 array. : High expression of was detected in three pancreatic cancer cell lines (SW1990, PANC-1, and AsPC-1). Knockdown of in SW1990 cells inhibited proliferation and enhanced apoptosis. : Our primary study suggests that might be involved in the progression of pancreatic cancer and may serve as a potential therapeutic target.
缺乏有效的治疗靶点是延长胰腺癌患者生存期和提高其生活质量面临的主要挑战。蛋白酶体26S亚基ATP酶2（ ）是最近发现的一个基因，已被证明与某些人类癌症发生有关。然而，关于 在癌细胞生长中的功能意义的数据有限，其是否在胰腺癌发生中起作用仍不清楚。：采用定量逆转录聚合酶链反应（qRT-PCR）评估 在不同胰腺癌细胞系中的mRNA表达水平。通过使用短发夹RNA（shRNA）实现 的敲低。通过MTT细胞增殖试验、Celigo试验、膜联蛋白V荧光激活细胞分选（FACS）试验和Caspase-3/7阵列评估 沉默对胰腺癌细胞增殖和凋亡的影响。：在三种胰腺癌细胞系（SW1990、PANC-1和AsPC-1）中检测到 的高表达。SW1990细胞中 的敲低抑制了增殖并增强了凋亡。：我们的初步研究表明， 可能参与胰腺癌的进展，并可能作为一个潜在的治疗靶点。
