环状 RNA circ_0000885 通过表观遗传地上调 Caprin1 促进肝细胞癌增殖。
Circular circ_0000885 promotes hepatocellular carcinoma proliferation by epigenetically upregulating Caprin1.
机构信息
Department of Infectious Disease, Zhangye People's Hospital Affiliated To Hexi University, Zhangye, China.
出版信息
Eur Rev Med Pharmacol Sci. 2019 Sep;23(18):7848-7854. doi: 10.26355/eurrev_201909_18994.
OBJECTIVE
Recently, the vital role of circular RNAs is discovered in many diseases, including tumor progression. Hepatocellular carcinoma (HCC) is one of the most ordinary malignant tumors. The purpose of our study is to detect the potential function of circ_0000885 in HCC to offer new biomarkers and targets.
PATIENTS AND METHODS
The expression level of circ_0000885 in HCC tissues and cell lines was monitored by Real Time-quantitative Polymerase Chain Reaction (RT-qPCR). Pearson's Chi-square test was used to determine the association of circ_0000885 expression with several clinicopathological factors. Then knockdown of circ_0000885 was constructed to uncover its function in HCC. The cell growth ability was measured through the cell counting kit-8 (CCK-8) assay, colony formation assay, and cell cycle assay. The Western blot assay was performed to analyze the protein level of Caprin1.
RESULTS
Circ_0000885 was highly expressed in HCC tissues than that in adjacent samples. The miR-532-5p expression was associated with lymphatic metastasis and TNM stage. The expression of circ_0000885 was also higher in HCC cell lines. The cell growth ability of HCC cells was inhibited after circ_0000885 was silenced. Furthermore, Caprin1 was inhibited via knockdown of circ_0000885.
CONCLUSIONS
Circ_0000885 could enhance cell proliferation and regulate cell cycle of HCC by promoting Caprin1.
目的
最近，环状 RNA 在许多疾病中，包括肿瘤进展中发挥着重要作用。肝细胞癌（HCC）是最常见的恶性肿瘤之一。我们的研究旨在检测 circ_0000885 在 HCC 中的潜在功能，为其提供新的生物标志物和靶点。
方法
通过实时定量聚合酶链反应（RT-qPCR）监测 HCC 组织和细胞系中 circ_0000885 的表达水平。采用皮尔逊卡方检验确定 circ_0000885 表达与几种临床病理因素的关系。然后构建 circ_0000885 的敲低，以揭示其在 HCC 中的功能。通过细胞计数试剂盒-8（CCK-8）测定、集落形成测定和细胞周期测定来测量细胞生长能力。通过 Western blot 测定分析 Caprin1 的蛋白水平。
结果
circ_0000885 在 HCC 组织中的表达高于相邻样本。miR-532-5p 的表达与淋巴转移和 TNM 分期有关。circ_0000885 在 HCC 细胞系中的表达也较高。沉默 circ_0000885 后，HCC 细胞的细胞生长能力受到抑制。此外，通过敲低 circ_0000885 抑制了 Caprin1。
结论
circ_0000885 通过促进 Caprin1 增强 HCC 细胞的增殖并调节细胞周期。
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