Department of Neurosciences, Biomedicine, and Movement Sciences, University of Verona, Policlinico G. B. Rossi, Verona, Italy.
Department of Neuroscience, Istituto Superiore di Sanità, Rome, Italy.
Ann Clin Transl Neurol. 2019 Oct;6(10):2120-2126. doi: 10.1002/acn3.50897. Epub 2019 Oct 10.
We applied RT-QuIC assay to detect α-synuclein aggregates in cerebrospinal fluid (CSF) of patients with suspected Creutzfeldt-Jakob disease who had a neuropathological diagnosis of dementia with Lewy bodies (DLB) (n = 7), other neurodegenerative diseases with α-synuclein mixed pathology (n = 20), or without Lewy-related pathology (n = 49). The test had a sensitivity of 92.9% and specificity of 95.9% in distinguishing α-synucleinopathies from non-α-synucleinopathies. When performed in the CSF of patients with DLB (n = 36), RT-QuIC was positive in 17/20 with probable DLB, 0/6 with possible DLB, and 0/10 with Alzheimer disease. These results indicate that RT-QuIC for α-synuclein is an accurate test for DLB diagnosis.
我们应用 RT-QuIC 检测法来检测疑似克雅氏病患者的脑脊液（CSF）中α-突触核蛋白聚集物，这些患者的神经病理学诊断为路易体痴呆（DLB）（n=7）、具有α-突触核蛋白混合病理学的其他神经退行性疾病（n=20）或无路易体相关病理学（n=49）。该检测在区分α-突触核蛋白病和非α-突触核蛋白病方面具有 92.9%的灵敏度和 95.9%的特异性。当在 DLB 患者的 CSF 中进行（n=36）时，RT-QuIC 在 20 例可能的 DLB 中阳性 17 例，6 例可能的 DLB 中为 0 例，10 例阿尔茨海默病中为 0 例。这些结果表明，用于检测α-突触核蛋白的 RT-QuIC 是 DLB 诊断的准确检测方法。
