Department of Pathogenic Biology, Hainan Medical University, Haikou, Hainan, China; Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, Hainan, China; Key Laboratory of Translation Medicine Tropical Diseases, Hainan Medical University, Haikou, Hainan, China.
Hainan Jingang Biological Technology Co., Ltd., Haikou, Hainan, China.
Acta Trop. 2020 Jan;201:105211. doi: 10.1016/j.actatropica.2019.105211. Epub 2019 Oct 7.
Enterocytozoon bieneusi is a potentially important zoonotic pathogen. However, there is no information on E. bieneusi infection of captive long-tailed macaques (Macaca fascicularis) in Hainan Province, China. Here 193 fecal specimens of M. fascicularis were collected from a breeding base in Hainan Province, China, housing non-human primates for experimental use. E. bieneusi was identified and genotyped by nested PCR analysis of the internal transcribed spacer (ITS) region of the rRNA gene. A total of 59 (30.6%) specimens were PCR-positive for E. bieneusi and 16 ITS genotypes were identified including nine known genotypes: Type IV (n = 19), D (n = 11), CM1 (n = 8), PigEBITS7 (n = 4), Pongo2 (n = 4), Peru8 (n = 3), Peru11 (n = 1), WL21 (n = 1) and CM2 (n = 1) and seven novel genotypes HNM-I to HNM-VII (one each). Importantly, genotypes D, Type IV, Peru8, PigEBITS7, and Peru11, which were the predominant (38/59, 64.4%) genotypes identified among captive M. fascicularis in this study, are also well-known human-pathogenic genotypes. All the genotypes of E. bieneusi identified here, including the seven novel ones, belonged to zoonotic Group 1. This is the first report of the identification of E. bieneusi in M. fascicularis in Hainan Province, China. The finding that the numerous known human-pathogenic types and seven novel genotypes of E. bieneusi all belong to zoonotic Group 1 indicates the possibility of transmission of this important pathogenic parasite between M. fascicularis and humans.
肠微孢子虫是一种重要的潜在人畜共患病病原体。然而，目前在中国海南省还没有关于圈养长尾猕猴（Macaca fascicularis）感染肠微孢子虫的信息。本研究从中国海南省的一个用于实验的非人类灵长类动物养殖基地收集了 193 份长尾猕猴粪便标本。通过对 rRNA 基因内转录间隔区（ITS）区域的嵌套 PCR 分析，鉴定和基因分型肠微孢子虫。共有 59 份（30.6%）标本 PCR 阳性，鉴定出 16 种 ITS 基因型，包括 9 种已知基因型：IV 型（n=19）、D 型（n=11）、CM1 型（n=8）、猪源肠微孢子虫 ITS7 型（n=4）、猩猩源 2 型（n=4）、秘鲁 8 型（n=3）、秘鲁 11 型（n=1）、WL21 型（n=1）和 CM2 型（n=1），以及 7 种新基因型 HNM-I 至 HNM-VII（各 1 种）。重要的是，本研究中圈养长尾猕猴主要（38/59，64.4%）鉴定的基因型 D、IV 型、秘鲁 8 型、猪源肠微孢子虫 ITS7 型和秘鲁 11 型也是众所周知的人类致病性基因型。本研究中鉴定的肠微孢子虫所有基因型，包括 7 种新基因型，均属于人畜共患病组 1。这是在中国海南省首次报道长尾猕猴感染肠微孢子虫。本研究发现，大量已知的人类致病性类型和 7 种新型基因型的肠微孢子虫均属于人畜共患病组 1，这表明这种重要的致病性寄生虫在长尾猕猴和人类之间传播的可能性。
