Department of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt.
Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Helwan University, Cairo, Egypt; Department of Functional Genome Analysis, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 580, Heidelberg, Germany.
Microb Pathog. 2020 Jan;138:103777. doi: 10.1016/j.micpath.2019.103777. Epub 2019 Oct 7.
The capacity of C. jejuni to survive acid and capture iron is a requirement for C. jejuni to colonize host and cause infection. Herein, we aimed to characterize the influence of iron on Campylobacter acid response. The capacity of C. jejuni to survive acid stress was greatly enhanced in presence of iron. Moreover, the acid stimulon of C. jejuni under iron-enriched condition was investigated using the microarray approach. A total of 211 genes were differentially expressed in C. jejuni. Differentially expressed genes were included in 21 functional groups that control Campylobacter physiology. C. jejuni induced expression of many genes that were previously shown to be important for Campylobacter acid survival such as flagella biogenesis genes and genes involved in cell envelope biogenesis. The microarray results were validated using RT-qPCR where there was a great similarity in data obtained by both techniques. Finally, comparative analysis with previous studies showed that acid exposure induced expression of many genes in C. jejuni that were not detected in other studies such as genes encoding for the heat shock proteins GroEL and GroES. Current data could help us understand the mechanism of C. jejuni acid survival and consequently overcome infection by this enteric pathogen.
空肠弯曲菌能够在酸性条件下存活并摄取铁，这是其在宿主体内定植并引起感染的必要条件。在此，我们旨在描述铁对空肠弯曲菌酸反应的影响。在铁存在的情况下，空肠弯曲菌耐受酸胁迫的能力大大增强。此外，我们还使用微阵列方法研究了富含铁条件下空肠弯曲菌的酸刺激物。在空肠弯曲菌中，共有 211 个基因的表达存在差异。差异表达的基因包含 21 个控制弯曲菌生理学的功能组。空肠弯曲菌诱导了许多先前被证明对弯曲菌酸存活很重要的基因的表达，如鞭毛生物发生基因和参与细胞包膜生物发生的基因。使用 RT-qPCR 对微阵列结果进行了验证，两种技术获得的数据非常相似。最后，与先前的研究进行比较分析表明，酸暴露诱导了空肠弯曲菌中许多基因的表达，而这些基因在其他研究中并未检测到，如编码热休克蛋白 GroEL 和 GroES 的基因。目前的数据可以帮助我们了解空肠弯曲菌酸存活的机制，从而克服这种肠道病原体的感染。
