Lin Shu-Hui, Chen Mu-Kuan, Chang Julia Hueimei, Velmurugan Bharath Kumar, Annamanedi Madhavi, Su Shih-Chi, Yeh Kun-Tu, Yang Shun-Fa
Department of Surgical Pathology, Changhua Christian Hospital, Changhua, Taiwan.
Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung, Taiwan.
J Cancer. 2019 Aug 28;10(21):5065-5069. doi: 10.7150/jca.34592. eCollection 2019.
In Taiwan, the incidence rate of oral cancer is constantly increasing. Polymorphisms and lifestyle habits are major contributing factors to the development of oral cancer in such cases. Casein kinase 1 epsilon () gene expression plays a role in numerous cancers, and the knockdown of induces tumor cell-selective cytotoxicity. The present study was designed to determine the effects of gene polymorphisms combined with environmental carcinogens on susceptibility to developing oral squamous cell carcinoma and its clinicopathological status. Four single-nucleotide polymorphisms (SNPs) in gene (rs135745, rs135764, rs1997644 and rs2075984) from 741 oral cancer patients and 462 healthy controls were analyzed using real-time polymerase chain reaction. Our results shown that variant types (GC) of polymorphic rs135745 exhibited a significantly higher risk of 1.41 (95% confidence interval [CI]: 1.036-1.919) for oral cancer than did wild type alleles. Furthermore, these gene SNPs along with betel-quid chewing and/or tobacco use further increased susceptibility to oral cancer. Moreover, variant genotypes (GC+CC) of CK1ε rs135745 were significantly associated with lymph node metastasis. These results suggested that the gene polymorphism is associated with the clinicopathological development of oral cancer and increases individuals' susceptibility to environmental carcinogens (e.g., smoking and betel-quid chewing) in terms of developing oral cancer.
在台湾，口腔癌的发病率持续上升。多态性和生活方式习惯是此类情况下口腔癌发生的主要促成因素。酪蛋白激酶1ε（CK1ε）基因表达在多种癌症中发挥作用，敲低CK1ε可诱导肿瘤细胞选择性细胞毒性。本研究旨在确定CK1ε基因多态性与环境致癌物联合作用对口腔鳞状细胞癌易感性及其临床病理状态的影响。采用实时聚合酶链反应分析了741例口腔癌患者和462例健康对照者CK1ε基因的4个单核苷酸多态性（SNP）（rs135745、rs135764、rs1997644和rs2075984）。我们的结果显示，CK1ε基因多态性rs135745的变异型（GC）患口腔癌的风险比野生型等位基因显著高1.41倍（95%置信区间[CI]：1.036 - 1.919）。此外，这些CK1ε基因SNP与嚼槟榔和/或吸烟进一步增加了患口腔癌的易感性。而且，CK1ε rs135745的变异基因型（GC + CC）与淋巴结转移显著相关。这些结果表明，CK1ε基因多态性与口腔癌的临床病理发展相关，并在患口腔癌方面增加了个体对环境致癌物（如吸烟和嚼槟榔）的易感性。
