Horký K, Gutkowska J, Garcia R, Thibault G, Genest J, Cantin M
Biochem Biophys Res Commun. 1985 Jun 28;129(3):651-7. doi: 10.1016/0006-291x(85)91941-2.
The effect of different conditions of blood withdrawal and use of different anesthetics on immunoreactive atrial natriuretic factor (IR-ANF) concentrations in plasma was studied in rats. The concentration of IR-ANF in plasma from jugular vein of non-anesthetized conscious rats, cannulated either 24 hr before blood withdrawal was 93.9 +/- 17.1 pg/ml (n = 30); and 48 hr: 81.9 +/- 11.5 pg/ml (n = 29). Immobilization stress (4 hr) increased IR-ANF concentration: 248.0 +/- 80.2 pg/ml (n = 5). Anesthesia by morphine, diethyl-ether, chloral hydrate and ketamine chlorhydrate increased IR-ANF concentrations to 2,443.0 +/- 281.2 pg/ml (n = 24), 806.1 +/- 74.6 pg/ml (n = 64), 224.0 +/- 81.4 pg/ml (n = 20), and 195.0 +/- 20.3 pg/ml (n = 51), respectively. IR-ANF in plasma of sodium-pentobarbital and urethane anesthetized rats was 59.2 +/- 6.7 pg/ml (n = 10) and 42.6 +/- 8.1 pg/ml (n = 8), respectively. These changes in IR-ANF evoked by different types of anesthetics and different conditions of blood withdrawal have to be taken into consideration during studies on the physiopathological role of atrial natriuretic factor.
研究了不同采血条件和使用不同麻醉剂对大鼠血浆中免疫反应性心房钠尿肽（IR-ANF）浓度的影响。未麻醉清醒大鼠在采血前24小时插管，从颈静脉采集的血浆中IR-ANF浓度为93.9±17.1 pg/ml（n = 30）；48小时时为81.9±11.5 pg/ml（n = 29）。固定应激（4小时）可增加IR-ANF浓度：248.0±80.2 pg/ml（n = 5）。吗啡、乙醚、水合氯醛和氯胺酮麻醉可使IR-ANF浓度分别升高至2443.0±281.2 pg/ml（n = 24）、806.1±74.6 pg/ml（n = 64）、224.0±81.4 pg/ml（n = 20）和195.0±20.3 pg/ml（n = 51）。戊巴比妥钠和乌拉坦麻醉的大鼠血浆中IR-ANF分别为59.2±6.7 pg/ml（n = 10）和42.6±8.1 pg/ml（n = 8）。在研究心房钠尿肽的生理病理作用时，必须考虑不同类型麻醉剂和不同采血条件引起的IR-ANF的这些变化。
