Institute of Food Science Research, CIAL (CSIC-UAM), Madrid, Spain.
Curr Pharm Des. 2019;25(32):3478-3483. doi: 10.2174/1381612825666191011094724.
In the last decade, various consortia and companies have created standardized digestion protocols and gastrointestinal simulators, such as the protocol proposed by the INFOGEST Consortium, the simulator SHIME, the simulator simgi®, the TIM, etc. Most of them claim to simulate the entire human gastrointestinal tract. However, few results have been reported on the use of these systems with potential prebiotic carbohydrates.
This critical review addresses the existing data on the analysis of prebiotic carbohydrates by different in vitro gastrointestinal simulators, the lack of parameters that could affect the results, and recommendations for their enhancement.
According to the reviewed data, there is a lack of a realistic approximation of the small intestinal conditions, mainly because of the absence of hydrolytic conditions, such as the presence of small intestinal brush border carbohydrases that can affect the digestibility of different carbohydrates, including prebiotics.
There is a necessity to standardize and enhance the small intestine simulators to study the in vitro digestibility of carbohydrates.
在过去的十年中，各种财团和公司已经创建了标准化的消化协议和胃肠道模拟器，例如 INFOGEST 财团提出的协议、SHIME 模拟器、simgi®模拟器、TIM 等。它们大多数声称可以模拟整个人体胃肠道。然而，很少有关于这些系统与潜在益生元碳水化合物一起使用的结果的报道。
本批判性评论解决了不同体外胃肠道模拟器分析益生元碳水化合物的现有数据、可能影响结果的参数缺失以及对其增强的建议。
根据审查的数据，缺乏对小肠条件的现实近似，主要是因为缺乏水解条件，例如小肠刷状缘碳水化合物酶的存在，这些酶可能会影响不同碳水化合物（包括益生元）的消化率。
有必要对小肠模拟器进行标准化和增强，以研究碳水化合物的体外消化率。
