Ferreira-Lazarte Alvaro, Olano Agustín, Villamiel Mar, Moreno F Javier
Instituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM) . C/Nicolás Cabrera, 9, Campus de la Universidad Autónoma de Madrid, 28049 Madrid, Spain.
J Agric Food Chem. 2017 Sep 13;65(36):8046-8053. doi: 10.1021/acs.jafc.7b01809. Epub 2017 Aug 29.
There are few studies on the assessment of digestibility of nondigestible carbohydrates, despite their increasingly important role in human health. In vitro digestibility of a range of dietary carbohydrates classified as digestible (maltose, sucrose, and lactose), well-recognized (lactulose, fructooligosaccharides (FOS), and two types of galactooligosaccharides (GOS) differing in the predominant glycosidic linkage), and potential (lactosucrose and GOS from lactulose, OsLu) prebiotics using a rat small intestinal extract (RSIE) under physiological conditions of temperature and pH is described. Recognized and potential prebiotics were highly resistant to RSIE digestion although partial hydrolysis at different extents was observed. FOS and lactulose were the most resistant to digestion, followed closely by OsLu and more distantly by both types of GOS and lactosucrose. In GOS, β(1 → 6) linkages were more resistant to digestion than β(1 → 4) bonds. The reported in vitro digestion model is a useful, simple, and cost-effective tool to evaluate the digestibility of dietary oligosaccharides.
尽管不可消化碳水化合物在人类健康中发挥着越来越重要的作用，但关于其消化率评估的研究却很少。本文描述了一系列被分类为可消化（麦芽糖、蔗糖和乳糖）、公认（乳果糖、低聚果糖（FOS）以及两种主要糖苷键不同的低聚半乳糖（GOS））和潜在（乳糖蔗糖以及由乳果糖制得的GOS，即OsLu）益生元的膳食碳水化合物在生理温度和pH条件下使用大鼠小肠提取物（RSIE）的体外消化率。公认的和潜在的益生元对RSIE消化具有高度抗性，尽管观察到了不同程度的部分水解。FOS和乳果糖对消化的抗性最强，其次是OsLu，两种GOS和乳糖蔗糖的抗性相对较弱。在GOS中，β(1→6)键比β(1→4)键对消化的抗性更强。所报道的体外消化模型是评估膳食低聚糖消化率的一种有用、简单且经济高效的工具。
