College of Life Sciences, Northeast Agricultural University, Harbin, 150030, China.
College of Life Science and Oceanography, Shenzhen University, Shenzhen, 518060, China.
Biochem Biophys Res Commun. 2019 Dec 3;520(2):311-319. doi: 10.1016/j.bbrc.2019.09.110. Epub 2019 Oct 8.
Indole glucosinolates are known to play essential and diverse roles in Arabidopsis immunity to pathogens. However, a complete understanding of the function of these compounds in plant immunity remains unclear. In this study, we investigated the transcriptome profile in loss-of-function mutant of MYB51, the key transcription factor that controls the biosynthesis of indole glucosinolates. Upon treatment with flg22 (a 22-amino acid peptide derived from bacterial flagellin), the genes that responded in a MYB51-dependent manner were analyzed. The results suggested that MYB51 was possibly implicated in most resistance processes, including pathogen recognition, signal transduction and PR protein activation. Of note, several genes in the ethylene pathway and the WRKY family, including WRKY33, were induced by flg22 in a MYB51-dependent manner. WRKY33 and ethylene were demonstrated to be crucial regulators in plant immunity defense and are functionally upstream of MYB51 during MAMP triggered immunity (MTI). This result suggested a "positive feedback loop" between MYB51 and its upstream regulators.
吲哚类硫代葡萄糖苷被认为在拟南芥对病原体的免疫中发挥着重要且多样的作用。然而，人们对这些化合物在植物免疫中的功能还没有完全了解。在这项研究中，我们研究了控制吲哚类硫代葡萄糖苷生物合成的关键转录因子 MYB51 功能丧失突变体的转录组图谱。在用 flg22（一种来源于细菌鞭毛的 22 个氨基酸肽）处理后，分析了以 MYB51 依赖的方式响应的基因。结果表明，MYB51 可能参与了大多数抗性过程，包括病原体识别、信号转导和 PR 蛋白的激活。值得注意的是，乙烯途径和 WRKY 家族的几个基因，包括 WRKY33，也被 flg22 以 MYB51 依赖的方式诱导。WRKY33 和乙烯被证明是植物免疫防御的关键调节剂，并且在 MAMP 触发免疫 (MTI) 期间是 MYB51 的功能上游调节剂。这一结果表明 MYB51 与其上游调节剂之间存在“正反馈回路”。
