Clay Nicole K, Adio Adewale M, Denoux Carine, Jander Georg, Ausubel Frederick M
Department of Genetics, Harvard Medical School, Massachusetts General Hospital, Boston, MA 02114, USA.
Science. 2009 Jan 2;323(5910):95-101. doi: 10.1126/science.1164627. Epub 2008 Dec 18.
The perception of pathogen or microbe-associated molecular pattern molecules by plants triggers a basal defense response analogous to animal innate immunity and is defined partly by the deposition of the glucan polymer callose at the cell wall at the site of pathogen contact. Transcriptional and metabolic profiling in Arabidopsis mutants, coupled with the monitoring of pathogen-triggered callose deposition, have identified major roles in pathogen response for the plant hormone ethylene and the secondary metabolite 4-methoxy-indol-3-ylmethylglucosinolate. Two genes, PEN2 and PEN3, are also necessary for resistance to pathogens and are required for both callose deposition and glucosinolate activation, suggesting that the pathogen-triggered callose response is required for resistance to microbial pathogens. Our study shows that well-studied plant metabolites, previously identified as important in avoiding damage by herbivores, are also required as a component of the plant defense response against microbial pathogens.
植物对病原体或微生物相关分子模式分子的感知会触发一种类似于动物先天免疫的基础防御反应，部分表现为在病原体接触部位的细胞壁上沉积葡聚糖聚合物胼胝质。对拟南芥突变体进行转录和代谢谱分析，并监测病原体触发的胼胝质沉积，已确定植物激素乙烯和次生代谢产物4-甲氧基吲哚-3-基甲基芥子油苷在病原体反应中起主要作用。两个基因PEN2和PEN3对病原体抗性也是必需的，并且在胼胝质沉积和芥子油苷激活过程中都需要，这表明病原体触发的胼胝质反应是抵抗微生物病原体所必需的。我们的研究表明，先前被确定在避免食草动物造成损害方面很重要的、经过充分研究的植物代谢产物，也是植物对抗微生物病原体防御反应的一个组成部分。
