State Key Laboratory of Crop Genetics & Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture and Rural Affairs of China, Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Ministry of Education of China, Nanjing Agricultural University, Nanjing 210095, China.
Nanjing Suman Plasma Engineering Research Institute, Nanjing Agricultural University, Nanjing 210095, China.
Int J Mol Sci. 2022 Jul 26;23(15):8222. doi: 10.3390/ijms23158222.
The transcription factor WRKY33 is a vital regulator of the biological process of the necrotrophic fungus (). However, its specific regulatory mechanism remains to be further investigated. In non-heading Chinese cabbage (NHCC, (syn. ) ssp. ), our previous study showed that BcWRKY33A is induced not only by salt stress, but also by infection. Here, we noticed that BcWRKY33A is expressed in trichomes and confer plant defense resistance. Disease symptoms and qRT-PCR analyses revealed that -overexpressing and -silencing lines were less and more severely impaired, respectively, than wild type upon treatment. Meanwhile, the transcripts' abundance of indolic glucosinolates' (IGSs) biosynthetic genes is consistent with plants' tolerance. Identification and expression pattern analysis of BcMYB51s showed that BcMYB51-3 has a similar trend to BcWRKY33A upon infection. Moreover, BcWRKY33A directly binds to the promoter, which was jointly confirmed by Y1H, dual-LUC, and EMSA assays. The importance of MYB51, the homolog of BcMYB51-3, in the BcWRKY33A-mediated resistance was also verified using the TRV-based VIGS system. Overall, our data concludes that BcWRKY33A directly activates the expression of and downstream IGSs' biosynthetic genes, thereby improving the tolerance of NHCC plants.
转录因子 WRKY33 是一种重要的调节因子，参与了坏死真菌的生物学过程。然而，其具体的调节机制仍需进一步研究。在不结球白菜（NHCC，（syn. ）ssp. ）中，我们之前的研究表明，BcWRKY33A 不仅受到盐胁迫的诱导，还受到 的诱导。在这里，我们注意到 BcWRKY33A 在毛状体中表达，并赋予植物防御抗性。疾病症状和 qRT-PCR 分析表明，与野生型相比，-过表达和 -沉默系在 处理后分别受到较小和更严重的损害。同时，吲哚葡萄糖苷（IGS）生物合成基因的转录本丰度与植物的 耐受性一致。BcMYB51s 的鉴定和表达模式分析表明，BcMYB51-3 在 感染后与 BcWRKY33A 具有相似的趋势。此外，BcWRKY33A 直接结合到 启动子上，这一结果通过 Y1H、双 LUC 和 EMSA 实验得到了共同证实。使用基于 TRV 的 VIGS 系统还验证了 MYB51（BcMYB51-3 的同源物）在 BcWRKY33A 介导的 抗性中的重要性。总之，我们的数据表明 BcWRKY33A 直接激活 和下游 IGSs 生物合成基因的表达，从而提高 NHCC 植物对 的耐受性。
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