School of Biology, Damghan University, Damghan, Iran.
Nutr Neurosci. 2021 Oct;24(10):747-761. doi: 10.1080/1028415X.2019.1674523. Epub 2019 Oct 14.
Alcohol exposure during pregnancy affects the developing fetus and causes a variety of physical and neurological abnormalities. Here we aim to study the effects of vitamin E on spatial learning and memory deficits and on changes in hippocampal brain-derived neurotrophic factor (BDNF) levels following perinatal ethanol exposure in rats. Pregnant Wistar rats received ethanol (4 g/kg) and vitamin E (doses of 100, 200, and 400 mg/kg) on day 0 of gestation (GD) until weaning (28 days). On postnatal days (PND) 29, the performance of spatial learning and memory of rats were measured using the Morris water maze (MWM). The expression of BDNF protein levels in the hippocampus was assayed using BDNF ELISA kits. Ethanol exposed group showed higher escape latency during training, reduced time spent in the target quadrant, higher escape location latency and average proximity in probe test. Vitamin E with doses of 100, 200 and 400 mg/kg significantly reduced escape latency during training. Also, vitamin E (400 mg/kg) significantly increased time spent in target quadrant, decreased escape location latency and average proximity in probe test. Maternal ethanol treatment significantly reduced the expression of BDNF protein in the hippocampus of offspring, whereas administration of vitamin E (400 mg/kg) significantly increased hippocampal BDNF in ethanol-treated rats. Vitamin E administration dose-dependently ameliorate learning and memory deficits induced by perinatal ethanol exposure and increased hippocampal BDNF levels. BDNF may be implicated in the beneficial effects of vitamin E on learning and memory in the perinatal ethanol-exposed rat.
孕期饮酒会影响胎儿发育，导致各种身体和神经发育异常。本研究旨在探讨维生素 E 对围生期乙醇暴露大鼠空间学习记忆缺陷及海马脑源性神经营养因子（BDNF）水平变化的影响。妊娠第 0 天（GD）至断奶（28 天），Wistar 孕鼠每天给予乙醇（4 g/kg）和维生素 E（100、200 和 400 mg/kg）。在出生后第 29 天，使用 Morris 水迷宫（MWM）测量大鼠的空间学习和记忆能力。使用 BDNF ELISA 试剂盒检测海马 BDNF 蛋白水平的表达。与对照组相比，乙醇暴露组在训练过程中表现出更高的逃避潜伏期，在目标象限花费的时间减少，在探测试验中逃避位置潜伏期和平均接近度增加。100、200 和 400 mg/kg 的维生素 E 可显著降低训练过程中的逃避潜伏期。此外，400 mg/kg 的维生素 E 可显著增加目标象限的时间，降低探测试验中的逃避位置潜伏期和平均接近度。母体乙醇处理显著降低了后代海马 BDNF 蛋白的表达，而维生素 E（400 mg/kg）的给药显著增加了乙醇处理大鼠海马 BDNF。维生素 E 的给药剂量依赖性地改善了围生期乙醇暴露引起的学习和记忆缺陷，并增加了海马 BDNF 水平。BDNF 可能参与了维生素 E 对围生期乙醇暴露大鼠学习和记忆的有益作用。
