在小鼠海马体中，表达小清蛋白和非特异性 GABA 能神经元的 GABA 释放被突触前 GABA 受体以不同的方式调节作用于 CA1 锥体神经元。

GABA releases from parvalbumin-expressing and unspecific GABAergic neurons onto CA1 pyramidal cells are differentially modulated by presynaptic GABA receptors in mouse hippocampus.

机构信息

Department of Anatomy, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, China; Research Center for Fluid Machinery Engineering and Technology, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.

Department of Neurology, University of Maryland School of Medicine, Baltimore, MD, 21201, USA.

出版信息

Biochem Biophys Res Commun. 2019 Dec 3;520(2):449-452. doi: 10.1016/j.bbrc.2019.10.044. Epub 2019 Oct 10.

DOI:10.1016/j.bbrc.2019.10.044

PMID:31607482

Abstract

Hippocampus CA1 pyramidal cells receive γ-aminobutyric acid (GABA) release from multiple GABAergic interneurons. Combining optogenetic strategy and whole-cell recordings, we demonstrate that baclofen, a specific GABA receptor agonist, depresses monosynaptic GABA receptor-mediated transmission from parvalbumin (PV)-expressing interneuron terminals onto pyramidal cells with less efficacy than that from the unspecific GABAergic terminals. The depression from PV neuron terminals is mainly mediated by presynaptic P/N type calcium channels. The results suggest that GABA receptors are widely expressed on GABAergic interneurons, where they exert inhibition onto pyramidal cells by GABA release with different efficacy. The data strengthen the proposal that diverse GABA neurons play different roles in modulating CA1 pyramidal cell excitability.

摘要

海马体 CA1 锥体神经元接收来自多种 GABA 能中间神经元的γ-氨基丁酸 (GABA) 释放。通过结合光遗传学策略和全细胞膜片钳记录，我们证明了巴氯芬，一种特定的 GABA 受体激动剂，对来自表达小清蛋白 (PV) 的中间神经元末梢的单突触 GABA 受体介导的传递的抑制作用不如非特异性 GABA 能末梢的抑制作用强。来自 PV 神经元末梢的抑制主要是通过突触前 P/N 型钙通道介导的。结果表明，GABA 受体广泛表达于 GABA 能中间神经元上，通过 GABA 释放以不同的效能对锥体细胞发挥抑制作用。这些数据支持了这样一种观点，即不同的 GABA 神经元在调节 CA1 锥体细胞兴奋性方面发挥着不同的作用。