Paetzold Claudia, Wood Kenneth R, Eaton Deren A R, Wagner Warren L, Appelhans Marc S
Department of Systematics, Biodiversity and Evolution of Plants (with Herbarium), University of Göttingen, Goettingen, Germany.
National Tropical Botanical Garden, Kalaheo, HI, United States.
Front Plant Sci. 2019 Sep 17;10:1074. doi: 10.3389/fpls.2019.01074. eCollection 2019.
Hawaiian are one of the major adaptive radiations of the Hawaiian Islands comprising 54 endemic species. The lineage is monophyletic with an estimated crown age predating the rise of the current high islands. Phylogenetic inference based on Sanger sequencing has not been sufficient to resolve species or deeper level relationships. Here, we apply restriction site-associated DNA sequencing (RAD-seq) to the lineage to infer phylogenetic relationships. We employ Quartet Sampling to assess information content and statistical support, and to quantify discordance as well as partitioned ABBA-BABA tests to uncover evidence of introgression. Our new results drastically improved resolution of relationships within Hawaiian . The lineage is divided into five fully supported main clades, two of which correspond to morphologically circumscribed infrageneric groups. We provide evidence for both ancestral and current hybridization events. We confirm the necessity for a taxonomic revision of the section , as well as a re-evaluation of several species complexes by combining genomic and morphological data.
夏威夷管舌鸟是夏威夷群岛主要的适应性辐射物种之一，包含54个特有物种。该谱系是单系的，估计其冠群年龄早于当前高岛的形成。基于桑格测序的系统发育推断不足以解决物种或更深层次的关系。在这里，我们对该谱系应用限制性位点关联DNA测序（RAD-seq）来推断系统发育关系。我们采用四重抽样来评估信息含量和统计支持，并量化不一致性，以及使用分区ABBA-BABA检验来揭示基因渗入的证据。我们的新结果极大地提高了夏威夷管舌鸟内部关系的分辨率。该谱系分为五个得到充分支持的主要分支，其中两个对应于形态学上界定的亚属组。我们提供了祖先杂交事件和当前杂交事件的证据。我们证实有必要对该属进行分类修订，以及通过结合基因组和形态学数据对几个物种复合体进行重新评估。
