Dong Yuqing, Wei Xueping, Qiang Tingyan, Liu Jiushi, Che Peng, Qi Yaodong, Zhang Bengang, Liu Haitao
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
Engineering Research Center of Tradition Chinese Medicine Resource, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
Front Plant Sci. 2022 Apr 26;13:857016. doi: 10.3389/fpls.2022.857016. eCollection 2022.
Most plants of have economic value and medicinal application. Among them, and its closely related species have been demonstrated to have definite efficacy. However, the taxonomy and phylogenetic relationship of in terms of morphology and commonly used gene regions remain controversial, which adversely affects its rational application. In this study, a total of 107 individuals of , , , , and were studied from the perspectives of genetic diversity, phylogeny, and ecology single nucleotide polymorphisms (SNPs) developed through restriction site-associated DNA sequencing (RAD-seq). Based on these SNPs, the genetic diversity, phylogenetic reconstruction, and population genetic structure were analyzed. Subsequently, divergence time estimation and differentiation scenario simulation were performed. Meanwhile, according to the species distribution records and bioclimatic variables, the Last Glacial Maximum and current potential distributions of five species were constructed, and the main ecological factors affecting the distribution of different species were extracted. The calculated showed that there was a moderate degree of differentiation among , , and , and there was a high degree of genetic differentiation between and the above species. The phylogenetic tree indicated that each of the species was monophyletic. The results of population genetic structure and divergence scenario simulation and D-statistics showed that there were admixture and gene flow among , , and . The results of ecological niche modeling indicated that the distribution areas and the bioclimatic variables affecting the distribution of and its related species were different. This study explored the differences in the genetic divergence and geographical distribution patterns of and its related species, clarifying the uniqueness of compared to its relatives and providing a reference for their rational application in the future.
大多数植物都具有经济价值和药用价值。其中，[植物名称]及其近缘物种已被证明具有一定疗效。然而，[植物名称]在形态学和常用基因区域方面的分类学和系统发育关系仍存在争议，这对其合理应用产生了不利影响。在本研究中，通过限制性位点相关DNA测序（RAD-seq）开发单核苷酸多态性（SNP），从遗传多样性、系统发育和生态学角度对[植物名称]、[植物名称]、[植物名称]、[植物名称]和[植物名称]的总共107个个体进行了研究。基于这些SNP，分析了遗传多样性、系统发育重建和群体遗传结构。随后，进行了分歧时间估计和分化情景模拟。同时，根据物种分布记录和生物气候变量，构建了五个物种的末次盛冰期和当前潜在分布，并提取了影响不同物种分布的主要生态因素。计算结果表明，[植物名称]、[植物名称]和[植物名称]之间存在中等程度的分化，[植物名称]与上述物种之间存在高度的遗传分化。系统发育树表明每个物种都是单系的。群体遗传结构、分歧情景模拟和D统计结果表明，[植物名称]、[植物名称]和[植物名称]之间存在混合和基因流。生态位建模结果表明，[植物名称]及其相关物种的分布区域和影响其分布的生物气候变量不同。本研究探讨了[植物名称]及其相关物种在遗传分化和地理分布模式上的差异，阐明了[植物名称]与其近缘种相比的独特性，并为其未来的合理应用提供了参考。
