Hishikawa Hidehiko, Kaibori Masaki, Tsuda Takumi, Matsui Kosuke, Okumura Tadayoshi, Ozeki Eiichi, Yoshii Kengo
Department of Surgery, Kansai Medical University, Hirakata, Osaka, Japan.
Technology Research Laboratory, Shimadzu Corporation, Kyoto, Japan.
Oncotarget. 2019 Sep 24;10(54):5622-5631. doi: 10.18632/oncotarget.27193.
The diagnostic use and therapeutic effect of near infrared fluorescence (NIF) imaging and photodynamic therapy (PDT) was investigated for gallbladder cancer using indocyanine green (ICG)-lactosomes. PDT was toxic for NOZ cells treated with ICG-lactosomes. Fluorescence intensity in the tumor region of mice administered ICG-lactosomes, but not ICG alone, was higher than the healthy contralateral region ≥24 hours after injection. PDT exerted immediate and continuous phototoxic effects in NOZ implanted mice injected with ICG-lactosomes. Enhanced antitumor effects were observed in the twice irradiated group compared with the once irradiated group. ICG or ICG-lactosomes were added to the human gallbladder cancer cell line NOZ followed by PDT and cell viability was measured. Mass spectrometry of ICG and ICG-lactosomes was performed after PDT. ICG or ICG-lactosomes were intravenously administered to BALB/c nude mice implanted subcutaneously with NOZ cells and fluorescence was evaluated by NIF imaging. Implanted tumors underwent PDT and antitumor effects were analyzed after performing irradiation once or twice in ICG-lactosome groups. ICG-lactosomes accumulated in xenograft tumors and PDT had an antitumor effect on these malignant tumors. NIF imaging with ICG-lactosomes and PDT may be useful diagnostic and/or therapeutic agents for gallbladder cancer.
使用吲哚菁绿（ICG）脂质体研究了近红外荧光（NIF）成像和光动力疗法（PDT）对胆囊癌的诊断用途及治疗效果。PDT对用ICG脂质体处理的NOZ细胞具有毒性。注射ICG脂质体而非单独注射ICG的小鼠，其肿瘤区域的荧光强度在注射后≥24小时高于对侧健康区域。PDT对注射了ICG脂质体的NOZ植入小鼠产生即时且持续的光毒性作用。与单次照射组相比，在两次照射组中观察到增强的抗肿瘤作用。将ICG或ICG脂质体添加到人胆囊癌细胞系NOZ中，随后进行PDT并测量细胞活力。PDT后对ICG和ICG脂质体进行质谱分析。将ICG或ICG脂质体静脉注射到皮下植入NOZ细胞的BALB/c裸鼠体内，并通过NIF成像评估荧光。对植入的肿瘤进行PDT，并在ICG脂质体组中进行一次或两次照射后分析抗肿瘤作用。ICG脂质体在异种移植肿瘤中蓄积，PDT对这些恶性肿瘤具有抗肿瘤作用。用ICG脂质体进行NIF成像和PDT可能是用于胆囊癌的有用诊断和/或治疗剂。
