Wen Liang, You Wendong, Wang Yadong, Zhu Yuanrun, Wang Hao, Yang Xiaofeng
Emergency and Trauma Center, The International Medical Center, The First Affiliated Hospital, School of Medicine, Zhejiang University.
Emergency and Trauma Center, The International Medical Center, The First Affiliated Hospital, School of Medicine, Zhejiang University;
J Vis Exp. 2019 Sep 19(151). doi: 10.3791/59410.
Increasing evidence shows that the microbiota-gut-brain axis plays an important role in the pathogenesis of brain diseases. Several studies also demonstrate that traumatic brain injuries cause changes to the gut microbiota. However, mechanisms underlying the bidirectional regulation of the brain-gut axis remain unknown. Currently, few models exist for studying the changes in gut microbiota after traumatic brain injury. Therefore, the presented study combines protocols for inducing traumatic brain injury using a lateral fluid percussion device and analysis of caecum samples following injury for investigating alterations in the gut microbiome. Alterations of the gut microbiota composition after traumatic brain injury are determined using 16S-rDNA sequencing. This protocol provides an effective method for studying the relationships between enteric microorganisms and traumatic brain injury.
越来越多的证据表明，微生物群-肠-脑轴在脑部疾病的发病机制中起重要作用。多项研究还表明，创伤性脑损伤会导致肠道微生物群发生变化。然而，脑-肠轴双向调节的潜在机制仍不清楚。目前，用于研究创伤性脑损伤后肠道微生物群变化的模型很少。因此，本研究结合了使用侧方流体冲击装置诱导创伤性脑损伤的方案以及损伤后对盲肠样本的分析，以研究肠道微生物组的变化。使用16S-rDNA测序确定创伤性脑损伤后肠道微生物群组成的变化。该方案为研究肠道微生物与创伤性脑损伤之间的关系提供了一种有效的方法。
