Department of Neurosurgery, Changzheng Hospital, Naval Medical University, China.
Department of Neurosurgery, Changzheng Hospital, Naval Medical University, China.
Brain Res. 2021 Jul 1;1762:147450. doi: 10.1016/j.brainres.2021.147450. Epub 2021 Mar 25.
Traumatic Brain Injury (TBI) present a significant burden to global health. Close association and mutual regulation exist between the brain and gut microbiota. In addition, metabolites may play an important role as intermediary mediators of the brain and gut microbiota. Consequently, the study sought to investigate the alterations in gut microbiota and metabolites after TBI and conducted a comprehensive analysis of the correlation between gut microbiota and metabolites after TBI in mice.
Changes in intestinal microbiota and metabolites in mice after moderate or severe traumatic brain injury were detected through 16S rDNA sequencing and the non-target LC-MS technology. Additionally, Pearson correlation analysis was used to explore the association between the microbiota and metabolites.
TBI was able to change the composition of intestinal microbiota, resulting to a decrease in microbial diversity in the intestinal tract (sham vs sTBI: 8.35 ± 0.12 vs 7.71 ± 0.5, p < 0.01; sTBI vs mTBI: 7.71 ± 0.5 vs 8.25 ± 0.34, p < 0.05). The results also showed that TBI could change the types and abundance of metabolites (723 in mTBI and sham groups; 1221 in sTBI and sham groups; 324 in mTBI and sTBI groups). Moreover, some of the altered gut metabolites were significantly correlated with part of the altered gut microbes after TBI.
TBI significantly changed intestinal microbiota as well as metabolites. Some of the altered microbiota and metabolites had a significant association. The results from this study provide information that paves way for future studies utilizing the brain gut axis theory in the diagnosis and treatment of TBI.
创伤性脑损伤（TBI）对全球健康造成了重大负担。大脑和肠道微生物群之间存在密切的关联和相互调节。此外，代谢物可能作为大脑和肠道微生物群的中介介质发挥重要作用。因此，本研究旨在探讨 TBI 后肠道微生物群和代谢物的变化，并对 TBI 后小鼠肠道微生物群和代谢物之间的相关性进行全面分析。
通过 16S rDNA 测序和非靶向 LC-MS 技术检测中度或重度创伤性脑损伤后小鼠肠道微生物群和代谢物的变化。此外，采用 Pearson 相关分析探讨微生物群和代谢物之间的相关性。
TBI 能够改变肠道微生物群的组成，导致肠道微生物多样性降低（假手术组 vs sTBI 组：8.35±0.12 对 7.71±0.5，p<0.01；sTBI 组 vs mTBI 组：7.71±0.5 对 8.25±0.34，p<0.05）。结果还表明，TBI 可以改变代谢物的类型和丰度（mTBI 和假手术组有 723 种；sTBI 和假手术组有 1221 种；mTBI 和 sTBI 组有 324 种）。此外，一些改变的肠道代谢物与 TBI 后部分改变的肠道微生物存在显著相关性。
TBI 显著改变了肠道微生物群和代谢物。一些改变的微生物群和代谢物之间存在显著的关联。本研究结果为利用脑肠轴理论在 TBI 的诊断和治疗中提供了信息。
