Laboratorio de Ecología Microbiana Medioambiental, Facultad de Química y Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
Laboratorio de Biología Computacional, Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Uruguay.
Int J Syst Evol Microbiol. 2020 Jan;70(1):562-568. doi: 10.1099/ijsem.0.003792.
An alkaliphilic, moderately halophilic, heterotrophic, rod-shaped, spore-forming bacterium (M30) was isolated from a sediment sample collected from a soda lake (Lake Magadi, Tanzania). Strain M30 was strictly aerobic, catalase-positive, oxidase-negative and non-motile. Growth occurred at 12-43 °C (optimum, 25-30 °C), at pH 8.0-12 (optimum, pH 9.5-10) and at salinities of 0.5-15 % (w/v) NaCl (optimum 5 %). It utilized various sugars and organic acids as sole carbon sources and was positive for amylase, cellulase, gelatinase, protease and xylanase activities. The cell-wall peptidoglycan contained -diaminopimelic acid and the polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified lipid and one unidentified phospholipid. The DNA G+C content was 48.9 mol%. The predominant menaquinone was MK-7 and the major fatty acids (>10 %) comprised anteiso-C, iso-C, and anteiso-C. Phylogenetic analysis based on 16S rRNA gene sequence affiliated M30 to the genus and showed the highest similarities to FJAT-45347 (96.4 %) and K1-5 (96.2 %). Based on the data from the current polyphasic study, M30 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M30 (=JCM 32118=CGMCC 1.16739=MCC 3010).
一株嗜碱、中度嗜盐、异养、杆状、产芽孢细菌（M30）从坦桑尼亚苏打湖（马加迪湖）采集的沉积物样本中分离得到。M30 菌株是严格需氧的，过氧化氢酶阳性，氧化酶阴性，不运动。在 12-43°C（最佳温度 25-30°C）、pH 8.0-12（最佳 pH 9.5-10）和 0.5-15%（w/v）NaCl（最佳 5%NaCl）的盐度下生长。它利用各种糖和有机酸作为唯一碳源，并且对淀粉酶、纤维素酶、明胶酶、蛋白酶和木聚糖酶活性呈阳性。细胞壁肽聚糖含有 -二氨基庚二酸，极性脂类由双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的脂类和一种未鉴定的磷脂组成。DNA G+C 含量为 48.9mol%。主要的menaquinone 是 MK-7，主要脂肪酸（>10%）包括 anteiso-C、iso-C 和 anteiso-C。基于 16S rRNA 基因序列的系统发育分析将 M30 归入 属，并与 FJAT-45347（96.4%）和 K1-5（96.2%）最为相似。基于当前多相研究的数据，M30 代表了 属的一个新种，建议使用 sp. nov. 作为其名称。模式菌株为 M30（=JCM 32118=CGMCC 1.16739=MCC 3010）。
