Vishnuvardhan Reddy Sultanpuram, Thirumala Mothe, Farooq Mohammed
Microbial Ecology Lab, Department of Biochemistry, Mahatma Gandhi University, Anneparthy, Yellareddygudem (PO), Nalgonda- 508254, Telangana, India.
Int J Syst Evol Microbiol. 2015 Aug;65(8):2441-2446. doi: 10.1099/ijs.0.000275. Epub 2015 Apr 23.
A novel Gram-stain-positive, rod-shaped, motile, endospore-forming and proteolytic bacterial strain, SPT, was isolated from Lonar soda lake, in India. On the basis of 16S rRNA gene sequence analysis it was identified as belonging to the class Firmibacteria and was most closely related to Bacillus cellulosilyticus DSM 2522T (96.7%) and other members of the genus Bacillus ( < 95.9%). Strain SPT was catalase- and oxidase-positive. The cell-wall peptidoglycan of strain SPT contained meso-diaminopimelic acid. Polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three phospholipids, two aminolipids and two unknown lipids. The predominant isoprenoid quinone was MK-7. Anteiso-C15 : 0 (26.8%) was the predominant fatty acid and significant proportions (>5%) of iso-C15 : 0 (20.9%), C16 : 1ω7c alcohol (6.3%), iso-C16 : 0 (6.3%) and anteiso-C17 : 0 (5.3 %) were also detected in strain SPT. The DNA G+C content of strain SPT was 38.9 mol%. The results of phylogenetic, chemotaxonomic and biochemical tests allowed a clear differentiation of strain SPT from all other members of the genus Bacillus. Strain SPT represents a novel member of the genus Bacillus, for which the name Bacilluscaseinilyticus sp. nov. is proposed. The type strain is SPT ( = MCC 2612T = JCM 30246T).
从印度的洛纳尔盐湖中分离出了一株新型革兰氏阳性、杆状、具运动性、产芽孢且能水解蛋白质的细菌菌株SPT。基于16S rRNA基因序列分析，它被鉴定为属于厚壁菌门，与解纤维素芽孢杆菌DSM 2522T（96.7%）及芽孢杆菌属的其他成员（<95.9%）关系最为密切。菌株SPT过氧化氢酶和氧化酶呈阳性。菌株SPT的细胞壁肽聚糖含有内消旋二氨基庚二酸。极性脂质包括双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、三种磷脂、两种氨基脂质和两种未知脂质。主要的类异戊二烯醌为MK-7。anteiso-C15:0（26.8%）是主要脂肪酸，在菌株SPT中还检测到了相当比例（>5%）的iso-C15:0（20.9%）、C16:1ω7c醇（6.3%）、iso-C16:0（6.3%）和anteiso-C17:0（5.3%）。菌株SPT的DNA G+C含量为38.9 mol%。系统发育、化学分类和生化测试结果使菌株SPT能够与芽孢杆菌属的所有其他成员明确区分开来。菌株SPT代表了芽孢杆菌属的一个新成员，为此提出了新名称解酪蛋白芽孢杆菌（Bacillus caseinilyticus sp. nov.）。模式菌株为SPT（=MCC 2612T =JCM 30246T）。
