Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2022 May;72(5). doi: 10.1099/ijsem.0.005351.
A Gram-stain-positive, aerobic, endospore-forming and rod-shaped bacterium (KQ-3), which grew at 10-45 °C (optimum 35 °C), pH 8.0-10.5 (optimum pH 9.0) and in the presence of 0-16 % (w/v) NaCl (optimum 3.0 %), was isolated from a soda lake and identified as representing a novel species using a polyphasic taxonomic approach. Strain KQ-3 was catalase-positive, oxidase-negative and non-motile. Phylogenetic analysis based on 16S rRNA gene sequence affiliated KQ-3 to the genus and showed the highest similarities to M30 (97.90 %), K1-5 (97.84 %) and FJAT-45347 (97.22 %). Digital DNA-DNA hybridization and average nucleotide identity analyses revealed that KQ-3 displayed 21.4 and 72.81% genomic DNA relatedness with the most closely related strain, M30, respectively. KQ-3 contained all of the conserved signature indels that are specific for members of the genus . The DNA G+C content was 45.03 mol%. The cell-wall peptidoglycan contained -diaminopimelic acid and the polar lipids consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and one unidentified phospholipid. The predominant menaquinone was MK-7 (100%) and the major fatty acids (>10 %) comprised anteiso-C, iso-C and iso-C. Based on the data from the current polyphasic studies, KQ-3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KQ-3 (=ACCC 61799=KCTC 33933).
一株革兰氏阳性、需氧、产芽孢的杆状细菌（KQ-3），可在 10-45°C（最适 35°C）、pH 8.0-10.5（最适 pH 9.0）和 0-16%（w/v）NaCl（最适 3.0%）的条件下生长，从苏打湖中分离得到，并采用多相分类方法鉴定为代表一个新种。KQ-3 菌株呈过氧化氢酶阳性、氧化酶阴性和非运动性。基于 16S rRNA 基因序列的系统发育分析将 KQ-3 归入 属，并与 M30（97.90%）、K1-5（97.84%）和 FJAT-45347（97.22%）最为相似。DNA-DNA 杂交和平均核苷酸同一性分析显示，KQ-3 与最密切相关的菌株 M30 的基因组 DNA 相似度分别为 21.4%和 72.81%。KQ-3 含有属内所有保守的特征缺失插入，其 DNA G+C 含量为 45.03mol%。细胞壁肽聚糖含有 -二氨基庚二酸，极性脂类由磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰甘油和一种未鉴定的磷脂组成。主要的menaquinone 为 MK-7（100%），主要脂肪酸（>10%）包括 anteiso-C、iso-C 和 iso-C。基于目前的多相研究数据，KQ-3 代表了 属的一个新种，建议将其命名为 sp. nov.。模式菌株为 KQ-3（=ACCC 61799=KCTC 33933）。
