心房利钠因子的放射免疫测定：人体血浆水平

Radioimmunoassay of atrial natriuretic factor: human plasma levels.

作者信息

Larose P, Meloche S, du Souich P, Deléan A, Ong H

出版信息

Biochem Biophys Res Commun. 1985 Jul 31;130(2):553-8. doi: 10.1016/0006-291x(85)90452-8.

Abstract

A simple and sensitive radioimmunoassay procedure has been developed for the determination of atrial natriuretic factor (ANF) in human plasma. The rabbit antiserum was obtained from a commercial source. ANF was extracted from plasma using an octadecasilyl silica cartridge with a recovery of 78.7%. HPLC of the plasma extract showed the presence of one immunoreactive peak of ANF corresponding to its low molecular weight form. Plasma ANF in humans increased from 8.0 +/- 2.2 in upright position to 20.0 +/- 5.9 fmol/ml (n = 6) in downward position (p less than 0.005).

摘要

已开发出一种简单且灵敏的放射免疫分析方法，用于测定人血浆中的心房利钠因子（ANF）。兔抗血清购自商业渠道。使用十八烷基硅烷硅胶柱从血浆中提取ANF，回收率为78.7%。血浆提取物的高效液相色谱显示存在一个与ANF低分子量形式相对应的免疫反应峰。人体血浆ANF在直立位时为8.0±2.2，在卧位时增加至20.0±5.9 fmol/ml（n = 6）（p＜0.005）。