University of Graz, Institute of Pharmaceutical Sciences, Department of Pharmacognosy, 8010GrazAustria.
University of Zagreb, Faculty of Science, Department of Biology (Botany), HR-10000Zagreb, Croatia.
Acta Pharm. 2019 Dec 1;69(4):673-681. doi: 10.2478/acph-2019-0037.
Little is known about the pharmacological activities of Iris adriatica (Iridaceae), a plant endemic to Dalmatia (Croatia). We therefore performed a bioassay-guided fractionation including high-performance counter current chromatography (HPCCC) and antibacterial tests using Mycobacterium smegmatis mc2 155. One obtained fraction was found to be antimycobacterially active with a MIC of 64 mg L-1. Furthermore, fractions were tested for resistance modulatory effects using ethidium bromide as substrate. We were able to identify the pure isoflavonic compounds irigenin and irilone and a fraction containing mainly benzophenone 2,4,6-trihydroxy-4-methoxy-benzophenone, responsible for the resistance-modulatory activity of this plant.
关于虹膜 Adriatica（鸢尾科）的植物，一种植物特有达尔马提亚（克罗地亚）的药理活性知之甚少。因此，我们进行了生物活性导向的分离，包括高效逆流色谱（HPCCC）和分枝杆菌分枝杆菌 mc2 155 抗菌试验。一个获得的分数被发现具有抗分枝杆菌活性，MIC 为 64 毫克 L-1。此外，还使用溴化乙锭作为底物测试了分数的耐药调节作用。我们能够鉴定出纯异黄酮化合物鸢尾素和 irilone 以及主要含有二苯甲酮 2,4,6-三羟基-4-甲氧基二苯甲酮的馏分，这是该植物耐药调节活性的原因。
