关于IgG抗IgG免疫复合物的组成
On the composition of IgG anti-IgG immune complexes.
作者信息
Steensgaard J, Johansen A S, Jacobsen C
出版信息
Immunology. 1979 Dec;38(4):697-704.
The formation of different immune complexes of IgG was followed in a system comprising human IgG as antigen and rabbit IgG directed against the Fc portion of human IgG as antibody. Soluble IgG complexes were analyzed by analytical zonal centrifugation. In antigen excess, 16S complexes predominated. 16S complexes are oligomers of IgG, mainly trimers and tetramers. In decreasing antigen excess larger and larger complexes were formed. It was, however, found consistently that oligomers were always formed in the largest amounts. The largest complexes detectable by this method consisted of about twenty IgG molecules. The solubility of different complexes in polyethylene glycol was also studied. Low concentrations of polyethylene glycol preferentially precipitate large complexes. Four and six per cent polyethylene glycol precipitated all types of IgG complexes although not completely. Polyethylene glycol was seemingly not bound directly to soluble immune complexes, but caued otherwise soluble complexes to precipitate by an indirect mechanism.
在一个以人IgG作为抗原、抗人IgG Fc段的兔IgG作为抗体的系统中，对不同的IgG免疫复合物的形成过程进行了跟踪研究。通过分析区带离心法对可溶性IgG复合物进行分析。在抗原过量时，16S复合物占主导。16S复合物是IgG的寡聚体，主要是三聚体和四聚体。随着抗原过量程度降低，形成的复合物越来越大。然而，一直发现寡聚体总是形成量最大。用该方法可检测到的最大复合物由约二十个IgG分子组成。还研究了不同复合物在聚乙二醇中的溶解度。低浓度的聚乙二醇优先沉淀大的复合物。4%和6%的聚乙二醇能沉淀所有类型的IgG复合物，尽管不完全。聚乙二醇似乎不是直接与可溶性免疫复合物结合，而是通过间接机制使原本可溶的复合物沉淀。
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