Department of Urology, Krankenhaus Hietzing mit Neurologischem Zentrum Rosenhügel, Vienna, Austria.
Department of Laboratory Medicine, Krankenhaus Hietzing mit Neurologischem Zentrum Rosenhügel, Vienna, Austria.
Andrologia. 2019 Dec;51(11):e13428. doi: 10.1111/and.13428. Epub 2019 Oct 22.
The effect of seminal leucocytes on sperm DNA integrity has been discussed controversially in literatures. Moreover, the studies investigating the in vivo effect of pro-inflammatory cytokines interleukin-6 and interleukin-8 on sperm DNA fragmentation are scarce and inconsistent. The association of standard sperm parameters with sperm DNA fragmentation is also a matter of ongoing discussion. Hence, the aims of this study were, first, to evaluate the effect of seminal leucocytes, interleukin-6 and interleukin-8 on sperm DNA integrity and, second, to examine whether standard semen parameters are associated with sperm DNA fragmentation. Seminal leucocytes, interleukin-6, interleukin-8 and standard semen parameters, including total sperm number, sperm concentration, progressive motility, nonprogressive motility, immotility and normal morphology, were determined in 134 consecutive men. The concentrations of seminal leucocytes, interleukin-6 and interleukin-8, did not correlate with sperm DNA fragmentation. In contrast, total sperm number, sperm concentration, progressive motility, nonprogressive motility and normal morphology exhibited significant inverse correlations with sperm DNA fragmentation. Immotile spermatozoa were directly correlated with sperm DNA fragmentation. In conclusion, seminal leucocytes, interleukin-6 and interleukin-8, are not associated with sperm DNA fragmentation. Poor standard semen parameters are significantly related to the high levels of sperm DNA fragmentation.
精液白细胞对精子 DNA 完整性的影响在文献中存在争议。此外，关于促炎细胞因子白细胞介素-6 和白细胞介素-8 对精子 DNA 碎片化的体内影响的研究很少且不一致。标准精子参数与精子 DNA 碎片化的相关性也是一个正在讨论的问题。因此，本研究的目的首先是评估精液白细胞、白细胞介素-6 和白细胞介素-8 对精子 DNA 完整性的影响，其次是检查标准精液参数是否与精子 DNA 碎片化相关。在 134 名连续男性中测定了精液白细胞、白细胞介素-6、白细胞介素-8 和标准精液参数，包括总精子数、精子浓度、前向运动精子百分率、非前向运动精子百分率、不动精子百分率和正常形态精子百分率。精液白细胞、白细胞介素-6 和白细胞介素-8 的浓度与精子 DNA 碎片化无相关性。相反，总精子数、精子浓度、前向运动精子百分率、非前向运动精子百分率和正常形态精子百分率与精子 DNA 碎片化呈显著负相关。不动精子与精子 DNA 碎片化呈直接相关。总之，精液白细胞、白细胞介素-6 和白细胞介素-8 与精子 DNA 碎片化无关。较差的标准精液参数与精子 DNA 碎片化水平升高显著相关。
