Evgeni E, Lymberopoulos G, Touloupidis S, Asimakopoulos B
Seminology Laboratory G. Lymberopoulos, Athens, Greece.
Laboratory of Physiology, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Thrace, Greece.
Andrologia. 2015 Dec;47(10):1166-74. doi: 10.1111/and.12398. Epub 2015 Jan 14.
Due to the limitations of conventional semen analysis in predicting a man's fertility potential, sperm DNA fragmentation was recently introduced as a novel marker of sperm quality. This prospective study was undertaken to investigate the associations between conventional seminal parameters and DNA fragmentation in Greek men. A total of 669 subject data were evaluated in two groups, normozoospermic (n = 184) and non-normozoospermic (n = 485), according to the WHO 2010 (WHO Laboratory Manual for the Examination and Processing of Human Semen, 5th edn. World Health Organization), reference limits. For all the subjects, semen volume, sperm concentration, total count, rapid and total progressive motility and morphology were recorded following the WHO 2010 methods and DNA fragmentation was assessed by the sperm chromatin dispersion assay. An inverse correlation was established between DNA fragmentation and all conventional seminal parameters except semen volume in men with seminal profiles below the reference limits, with statistical significance for rapid and total progressive motility. Normozoospermic men exhibited lower levels of DNA fragmentation than their non-normozoospermic counterparts, even though the values were not always below 30%. DNA fragmentation testing and traditional semen analysis should therefore be considered as complementary diagnostic tools in a comprehensive evaluation of male infertility.
由于传统精液分析在预测男性生育潜力方面存在局限性，精子DNA碎片化最近被引入作为精子质量的一个新指标。本前瞻性研究旨在调查希腊男性传统精液参数与DNA碎片化之间的关联。根据世界卫生组织2010年（《世界卫生组织人类精液检查与处理实验室手册》第5版）参考限值，将669名受试者的数据分为两组进行评估，即正常精子症组（n = 184）和非正常精子症组（n = 485）。对所有受试者，按照世界卫生组织2010年的方法记录精液体积、精子浓度、总数、快速和总渐进性活力以及形态，并通过精子染色质扩散试验评估DNA碎片化。在精液参数低于参考限值的男性中，除精液体积外，DNA碎片化与所有传统精液参数之间均呈负相关，快速和总渐进性活力具有统计学意义。正常精子症男性的DNA碎片化水平低于非正常精子症男性，尽管这些值并不总是低于30%。因此，在男性不育的综合评估中，DNA碎片化检测和传统精液分析应被视为互补的诊断工具。
