Fortier M A, Guilbault L A, Grasso F
Centre de Recherches en Endocrinologie Moléculaire, Centre Hospitalier de l'Université Laval, Ste-Foy, Quebec, Canada.
J Reprod Fertil. 1988 May;83(1):239-48. doi: 10.1530/jrf.0.0830239.
Epithelial and stromal cells were isolated from endometrium of cyclic heifers by enzymic dispersion. These cells exhibited specific morphological and functional properties. Epithelial cells appeared cuboidal or columnal and showed contact inhibition as they reached confluence. Stromal cells were fibroblast-like and enlarged at the time of confluence after which they overgrew in multiple layers. The presence of specific receptors for PGE-2 and beta-adrenergic catecholamines (isoproterenol) was estimated by activation of adenylate cyclase. Stromal cells had more adenylate cyclase activity (P less than 0.01) than did epithelial cells before (basal) and after stimulation with guanosine triphosphate (GTP) and PGE-2. However, epithelial cells were much more responsive to isoproterenol (P less than 0.01). Treatment of cultured cells with indomethacin to block PG synthesis increased the sensitivity and maximal response to PGE-2 in stromal (P less than 0.01) but not in epithelial (P greater than 0.1) cells. The latter result suggested autologous desensitization of the PGE-2 response resulting from synthesis of PGs in cultured cells. Both cell types synthesized PGs in culture: PGF-2 alpha was synthesized in greater quantity in epithelial than in stromal cells (P less than 0.05) while stromal cells synthesized more PGE-2 than did epithelial cells (P less than 0.001). Endometrial cells separated in this way should prove useful for study of their specific role in the processes of implantation and maternal recognition of pregnancy.
通过酶解分散法从周期性小母牛的子宫内膜中分离出上皮细胞和基质细胞。这些细胞呈现出特定的形态和功能特性。上皮细胞呈立方形或柱状，汇合时表现出接触抑制。基质细胞呈成纤维细胞样，汇合时体积增大，之后会多层过度生长。通过腺苷酸环化酶的激活来评估前列腺素E-2（PGE-2）和β-肾上腺素能儿茶酚胺（异丙肾上腺素）特异性受体的存在情况。在鸟苷三磷酸（GTP）和PGE-2刺激之前（基础状态）及之后，基质细胞的腺苷酸环化酶活性比上皮细胞更高（P<0.01）。然而，上皮细胞对异丙肾上腺素的反应更为敏感（P<0.01）。用吲哚美辛处理培养细胞以阻断前列腺素合成，可增加基质细胞（P<0.01）对PGE-2的敏感性和最大反应，但上皮细胞（P>0.1）则无此现象。后一结果提示培养细胞中前列腺素合成导致PGE-2反应的自身脱敏。两种细胞类型在培养中均合成前列腺素：上皮细胞中合成的前列腺素F-2α（PGF-2α）比基质细胞更多（P<0.05），而基质细胞合成的PGE-2比上皮细胞更多（P<0.001）。以这种方式分离的子宫内膜细胞对于研究它们在着床和母体对妊娠的识别过程中的特定作用应是有用的。
